Recombinant Human Xylulokinase/XYLB Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8267-XB

R&D Systems, part of Bio-Techne
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8267-XB-020
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Key Product Details

Source

E. coli

Accession #

O75191

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Xylulokinase/XYLB Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Xylulokinase/XYLB protein
Ala2-Glu536, with an N-terminal Met and 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

59 kDa

SDS-PAGE

56 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate D-Xylulose.
The specific activity is >6,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8267-XB
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Xylulokinase/XYLB

D-Xylulokinase (XYLB) catalyzes the phosphorylation of D-xylulose (Xu) to produce xylulose-5-phosphate (Xu5P) mainly in liver and kidney and is inhibited by 5‑deoxy‑5‑fluoro‑D‑xylulose (1). XYLB is the last enzyme in the glucuronate-xylulose pathway, and its product Xu5P interfaces with the pentose phosphate pathway. XYLB belongs to a family of enzymes that also include fucokinase, gluconokinase and glycerokinase. Recently, the metabolic pathway has re-emerged as a focus for drug discovery in cancer research as rapidly growing malignant tumor cells typically have glycolytic rates up to 200 times higher than those of their normal tissues of origin, i.e. the Warburg effect (2, 3). Metabolic enzymes, such as PKM2 (4) and phosphofructokinase 1 (5), are being explored as drug targets. Given that Xu5P is a key regulator of glucose metabolism and lipogenesis (1), XYLB could also be an attractive drug target. The kinase activity was measured using a phosphatase-coupled method (6).

References

  1. Bunker, R.D. et al. (2013) J. Biol. Chem. 288:1643.
  2. Gatenby, R.A. and Gillies, R.J. (2004). Nat. Rev. Cancer 4:891.
  3. Kim, J.W. and Dang, C.V. (2006). Cancer Res. 66:8927.
  4. Israelsen, W.J. et al. (2013) Cell 155:397.
  5. Yi, W. et al. (2012) Science 337:975.
  6. Wu, Z. (2011) PLoS ONE 6:e23172.

Alternate Names

XYLB, Xylulose Kinase

Entrez Gene IDs

9942 (Human); 102448 (Mouse); 316067 (Rat)

Gene Symbol

XYLB

UniProt

O75191

Additional Xylulokinase/XYLB Products

Product Documents for Recombinant Human Xylulokinase/XYLB Protein, CF

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Product Specific Notices for Recombinant Human Xylulokinase/XYLB Protein, CF

For research use only

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