Recombinant Influenza A Virus H5N1 Neuraminidase Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 7597-NM
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived influenza a virus h5n1 Viral Neuraminidase protein
Ser37-Lys449, with an N-terminal vasodilator-stimulated phosphoprotein tetramerization domain and a C-terminal 6-His tag
Ser37-Lys449, with an N-terminal vasodilator-stimulated phosphoprotein tetramerization domain and a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser (tetramerization domain)
Predicted Molecular Mass
51 kDa
SDS-PAGE
58-65 kDa, reducing conditions
Activity
Measured by its ability to cleave a fluorogenic substrate, 2’-(4-Methylumbelliferyl)-alpha -D-N-acetylneuraminic acid.
The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.
The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
7597-NM
Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Viral Neuraminidase
References
- Palese, P. & Compans, R. W. (1976) J. Gen. Virol. 33:159.
- Colman, P. M. et al. (1983) Nature 303:41.
- Wu, Z.L. et al. (2009) Biochem. Biophys. Res. Commun. 379:749.
- Sun, S. et al. (2012) PLoS one 7:e32119.
- Russell, R.J. et al. (2006) Nature 443:45.
- Govorkova, E.A. et al. (2005) J. Virol. 79:2191.
- Ducatez, M.F. et al. (2006) Nature 442:37.
- Peiris, J.S. et al. (2007) Clin. Microbiol. Rev. 20:243.
- http://www.who.int/influenza/human_animal_interface/EN_GIP_20120405NCumulativeNumberH5N1cases.pdf
- Kuhnel, K. et al. (2004) Proc. Natl. Acad. Sci. U. S. A. 101:17027.
Long Name
Neuraminidase
Alternate Names
NANH
UniProt
Additional Viral Neuraminidase Products
Product Documents for Recombinant Influenza A Virus H5N1 Neuraminidase Protein, CF
Product Specific Notices for Recombinant Influenza A Virus H5N1 Neuraminidase Protein, CF
Coomassie is a registered trademark of Imperial Chemical Industries Ltd.
For research use only
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