Recombinant M. viridifaciens Neuraminidase Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5084-NM

R&D Systems, part of Bio-Techne
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5084-NM-010
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

E. coli

Accession #

BAA00852

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Bacterial Neuraminidase Proteins and Enzymes »

Product Specifications

Source

E. coli-derived m. viridifaciens Bacterial Neuraminidase protein
Pro42-Gly403, with an N-terminal Met and 6-His tag
Accession # BAA00852

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

40 kDa

SDS-PAGE

42 kDa, reducing conditions

Activity

Measured by its ability to cleave a fluorogenic substrate, 2’-(4-Methylumbelliferyl)-alpha -D-N-acetylneuraminic acid.
The specific activity is >25,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

5084-NM
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Bacterial Neuraminidase

Neuraminidase is the common name for N-acetyl-neuraminyl hydrolase (Sialidase). Micromonospora viridifaciens Neuraminidase catalyzes the hydrolysis of alpha2-3 and alpha2-6 and alpha2-8 linked N-acetyl-neuraminic acid residues from glycoproteins and glycolipids (1). The architecture of the full-length protein includes a canonical neuraminidase enzymatic domain, a linker domain and a C-terminal galactose binding domain (2). The full-length protein contains 647 amino acids and has a molecular weight of 68 kDa. It is easily degraded to 52 kDa and 41 kDa fragments (3). The expressed enzyme only contains the enzymatic domain.

References

  1. Aisaka, K. & Uwajima, T. (1987) FEBS Microbiol. Lett. 44:289.
  2. Gaskell, A. et al. (1995) Structure 3:1197.
  3. Sakurada, K. et al. (1992) J. Bacteriol. 174:6896.

UniProt

BAA00852

Additional Bacterial Neuraminidase Products

Product Documents for Recombinant M. viridifaciens Neuraminidase Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant M. viridifaciens Neuraminidase Protein, CF

For research use only

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