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Recombinant Mouse Active JNK1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1776-KS

R&D Systems, part of Bio-Techne
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1776-KS-010

Key Product Details

Source

Sf 9 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 9 (baculovirus)-derived mouse JNK1 protein

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Using an N terminal GST tag

SDS-PAGE

70 kDa

Activity

The specific activity of JNK1 was determined to be 104 nmol/min/mg using an ATF2 substrate and was equivalent to 122 nmol/min/mg as per radiometric assay.

Scientific Data Images for Recombinant Mouse Active JNK1 Protein, CF

Recombinant Mouse Active JNK1 Protein SDS-PAGE

Recombinant Mouse Active JNK1 Protein SDS-PAGE.

The approximate molecular weight is 70 kDa and the purity is > 90%.

Formulation, Preparation and Storage

1776-KS
Formulation Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: JNK1

JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation on Threonine and Tyrosine residues during exposure to stress such as UV irradiation. JNK1 binds to the c-Jun transactivation domain and phosphorylates it on S63 and S73 (1). JNK1 has been shown to play an important role in disease processes. Activation of JNK1 results in defects in myotube viability and integrity leading to dystrophic myofiber destruction (2). JNK1 activity is also abnormally elevated in obesity and removal of JNK1 results in decreased adiposity and significantly improved insulin sensitivity.

References

  1. Derijard, B. et al. (1994) Cell 76:1025.
  2. Kolodziejczyk, S.M. et al. (2001) Curr. Biol. 11:1278.

Long Name

C-Jun N-terminal Kinase 1

Alternate Names

MAPK8, PRKM8, SAPK1

Entrez Gene IDs

5599 (Human); 26420 (Mouse); 116554 (Rat)

Gene Symbol

MAPK8

UniProt

Additional JNK1 Products

Product Documents for Recombinant Mouse Active JNK1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Active JNK1 Protein, CF

For research use only

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