Recombinant Mouse Active Trypsin 3/PRSS3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7235-SE

R&D Systems, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

NP_035775

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Trypsin 3/PRSS3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse Trypsin 3/PRSS3 protein
Ile24-Asn246, with an N-terminal Met, 6-His tag and GGSGSGGGSSR linker

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile24

Predicted Molecular Mass

24 kDa

SDS-PAGE

24-27 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >30,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7235-SE
Formulation Supplied as a 0.2 μm filtered solution in Citrate, NaCl, CaCl2, Benz, DTT, and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Trypsin 3/PRSS3

Mouse Trypsin 3, encoded by the Prss3 gene, is also known as mesotrypsin and brain trypsin (1). It is one of three trypsin isoforms produced by the pancreas (2). It is secreted into the duodenum lumen, where it is activated by enterokinase. Trypsin 3 differs from Trypsin 1 and 2 because it is resistant to polypeptide trypsin inhibitors such as the Kunitz-type soybean trypsin inhibitor and the Kazal‑type pancreatic secretory trypsin inhibitor (3). It has been proposed that a function of Trypsin 3 is the degradation of trypsin inhibitors to facilitate the digestion of foods rich in these proteins (4). Recombinant mouse Trypsin 3 is expressed and processed from E.coli, which yields the purified mature chain of mouse Trypsin 3.

References

  1. Nyarahucha, C.N.M. et al. (1997) J. Biol. Chem. 272:10573.
  2. Rinderknecht, H. et al. (1984) Gastroenterology 86:681.
  3. Katona, G. et al. (2002) J. Mol. Biol. 315:1209.
  4. Szmola, R. et al. (2003) J. Biol. Chem. 278:48580.

Alternate Names

Mesotrypsin, MTG, PRSS3, PRSS4, Trypsin 4, Trypsinogen 15

Entrez Gene IDs

5646 (Human); 22073 (Mouse); 362347 (Rat)

Gene Symbol

PRSS3

UniProt

NP_035775

Additional Trypsin 3/PRSS3 Products

Product Documents for Recombinant Mouse Active Trypsin 3/PRSS3 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse Active Trypsin 3/PRSS3 Protein, CF

For research use only

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