Recombinant Mouse Activin RIA/ALK-2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6506-RA

R&D Systems, part of Bio-Techne
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6506-RA-100
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Key Product Details

Source

NS0

Accession #

P37172

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Activin RIA/ALK-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Activin RIA/ALK-2 protein
Mouse Activin RIA
(Met1-Glu123)
Accession # P37172		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp23

Predicted Molecular Mass

38.4 kDa (monomer)

SDS-PAGE

45-50 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Activin RIA Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human BMP‑6 (Catalog # 507-BP) that produces 50% of the optimal binding response is found to be 6-60 ng/mL.

Formulation, Preparation and Storage

6506-RA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIA/ALK-2

Activin RIA, also known as ALK-2, TSK-7L, SKR1, TSR-I, and ACTR-I, is a glycosylated 65 kDa type I receptor in the TGF-beta serine/threonine kinase receptor family (1-3). Binding of TGF-beta superfamily ligands induces formation of a heterotetrameric complex that contains two chains each of a type I and a type II receptor in multiple combinations. The type II receptors phosphorylate the type I receptors which then phosphorylate and activate Smad signal transduction proteins (1, 2). Activin RIA functions in a wide variety of growth and differentiation processes including gastrulation, skeletal system development, and cardiac morphogenesis (1 4-7). Mature mouse Activin RIA consists of a 103 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 363 aa cytoplasmic region that includes a Gly/Ser-rich GS box and the kinase domain (8). Within the ECD, mouse Activin RIA shares 99% and 93% aa sequence identity with human and rat Activin RIA, respectively. It shares 24%-26% aa sequence identity with other mouse type I receptors Activin RIB, BMPR-IA, BMPR-IB, and TGF-beta RI. BMP signaling through Activin RIA is enhanced by the direct interaction between Activin RIA and RGM-B/DRAGON, a BMP coreceptor that also associates with other type I and type II receptors (9). Activin RIA can additionally phosphorylate the co‑receptor Endoglin and blocks the inhibitory effect of Endoglin on prostate cancer cell motility (10).

References

  1. Wu, M.Y. and C.S. Hill (2009) Dev. Cell 16:329.
  2. Nickel, J. et al. (2009) Cytokine Growth Factor Rev. 20:367.
  3. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  4. Chuva de Sousa Lopes, S.M. et al. (2004) Genes Dev. 18:1838.
  5. Zhang, D. et al. (2003) J. Bone Miner. Res. 18:1593.
  6. van Dinther, M. et al. (2010) J. Bone Miner. Res. 25:1208.
  7. Wang, J. et al. (2005) Dev. Biol. 286:299.
  8. Ebner, R. et al. (1993) Science 260:1344.
  9. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
  10. Romero, D. et al. (2010) Carcinogenesis 31:359.

Long Name

Activin Receptor IA

Alternate Names

ActivinRIA, ACVR1, ALK-2, ALK2

Entrez Gene IDs

90 (Human); 11477 (Mouse)

Gene Symbol

ACVR1

UniProt

P37172

Additional Activin RIA/ALK-2 Products

Product Documents for Recombinant Mouse Activin RIA/ALK-2 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse Activin RIA/ALK-2 Fc Chimera Protein, CF

For research use only

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