Recombinant Mouse Activin RIIA Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6356-R2
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Activin RIIA protein
Mouse Activin RIIA (Met1 - Pro134) Accession # P27038 |
IEGRMDP | Mouse IgG2A (Glu98 - Lys330) |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala20
Predicted Molecular Mass
40.5 kDa (monomer)
SDS-PAGE
60-65 kDa, reducing conditions
Activity
Measured by its ability to inhibit Activin A-induced hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340.
The ED50 for this effect is 6-36 ng/mL in the presence of 3 ng/mL of recombinant human Activin A (Catalog # 338-AC).
The ED50 for this effect is 6-36 ng/mL in the presence of 3 ng/mL of recombinant human Activin A (Catalog # 338-AC).
Reviewed Applications
Read 1 review rated 5 using 6356-R2 in the following applications:
Formulation, Preparation and Storage
6356-R2
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 100 μg/mL in PBS.
|
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Activin RIIA
References
- Welt, C. et al. (2002) Exp. Biol. Med. 227:724.
- Chen, Y.-G. et al. (2006) Exp. Biol. Med. 231:534.
- Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
- de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
- Mathews, L.S. and W.W. Vale (1991) Cell 65:973.
- Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
- Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
- Lewis, K.A. et al. (2000) Nature 404:411.
- Onichtchouk, D. et al. (1999) Nature 401:480.
- Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
- Shoji, H. et al. (2000) J. Biol. Chem. 275:5485.
- Matsuzaki, T. et al. (2002) J. Biol. Chem. 277:19008.
Long Name
Activin Receptor IIA
Alternate Names
ActivinRIIA, ACVR2A, AVR2A
Gene Symbol
ACVR2A
UniProt
Additional Activin RIIA Products
Product Documents for Recombinant Mouse Activin RIIA Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse Activin RIIA Fc Chimera Protein, CF
For research use only
Loading...
Loading...
Loading...