Recombinant Mouse Agrin His-tag Protein, CF

Name: Recombinant Mouse Agrin His-tag Protein, CF
Brand: R&D Systems
SKU: 11573-AG

R&D Systems, part of Bio-Techne | Catalog # 11573-AG

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Key Product Details

Accession #:	A2ASQ1.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Agrin His-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Mouse Agrin His-tag Protein, CF

N-terminal sequence Analysis	His (of 6-His tag)
Predicted Molecular Mass	105 kDa
SDS-PAGE	105 kDa

Scientific Data Examples for Recombinant Mouse Agrin His-tag Protein, CF

	Recombinant Mouse Agrin His-tag Protein Binding Activity. Measured by its binding ability in a functional ELISA. Recombinant Mouse Agrin His-tag Protein (Catalog # 11573-AG) binds to Recombinant Mouse LRP-4 His-tag Protein (10229-LR) with an ED₅₀ of 30.0‑300 ng/mL.
	Recombinant Mouse Agrin His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Mouse Agrin His-tag Protein (Catalog # 11573-AG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 114-128 kDa under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11573-AG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 1.00 mg/mL in PBS.
Stability & Storage:	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Agrin

Agrin is a 400‑600 kDa heparan sulfate proteoglycan component of the extracellular matrix. The N‑terminal half of mouse Agrin, which mediates ECM interactions, contains nine Kazal-type protease inhibitor domains, two Laminin EGF-like domains, and one SEA domain. The C-terminal half contains four EGF-like repeats and three Laminin globular G domains. Human Agrin also contains a Laminin‑binding N-terminal Agrin domain (NtA), and mouse and chick Agrin include the NtA domain only by the use of an alternate promoter. Additional isoforms are generated by alternate splicing at sites Y and Z in the C-terminal half of rat Agrin (known as A and B, respectively in chick). Agrin isoforms that contain an insert at site Z (Z+ forms) are known as neural Agrin and are selectively produced by motoneurons. Other isoforms are known as muscle Agrin and are additionally expressed in non-neuronal tissues, particularly in basement membranes of the lung and kidney (1-3). This recombinant protein consists of the C-terminal half of mouse Agrin. It shares 59%, 82%, and 94% aa sequence identity with comparable regions of chick, human, and rat Agrin, respectively. The C-terminal half of Z- and Z+ Agrin binds to alpha -Dystroglycan and mediates adhesion between motoneurons and myotubes at the neuromuscular junction (NMJ) (4-6). In contrast, only Z+ Agrin is effective at inducing clustering of the postsynaptic Acetylcholine Receptor (AChR) and presynaptic motoneuron differentiation (7, 8). Agrin‑induced AChR clustering requires a myotube receptor complex that contains alpha -Dystroglycan, MuSK, and LRP4 (4, 9-11). Agrin exhibits many functions in addition to NMJ development. It is enriched in senile Alzheimer’s disease plaques where it binds the A beta (1-40) peptide and promotes amyloid fibril formation (12). It regulates neuronal excitability by binding and inhibiting the alpha 3 subunit of the neuronal Na/K ATPase (13). It functions as an epithelial cell attachment receptor for HIV-1 through interactions with the gp41 coat protein (14). During T cell activation, Agrin contributes to formation of the immunological synapse and regulates the threshold of T cell activation (15).

Item	Value
References	Jury, E.C. and P.S. Kabouridis (2010) Arthritis Res. Ther. 12:205. Bezakova, G. and M.A. Ruegg (2003) Nat. Rev. Mol. Cell Biol. 4:295. Rupp, F. et al. (1991) Neuron 6:811. Gee, S.H. et al. (1994) Cell 77:675. Sugiyama, J. et al. (1994) Neuron 13:103. Gesemann, M. et al. (1998) J. Biol. Chem. 273:600. Burgess, R.W. et al. (1999) Neuron 23:33. Ferns, M.J. et al. (1993) Neuron 11:491. Glass, D.J. et al. (1996) Cell 85:513. Kim, N. et al. (2008) Cell 135:334. Zhang, B. et al. (2008) Neuron 60:285. Cotman, S.L. et al. (2000) Mol. Cell. Neurosci. 15:183. Hilgenberg, L.G.W. et al. (2006) Cell 125:359. Alfsen, A. et al. (2005) Mol. Biol. Cell 16:4267. Khan, A.A. et al. (2001) Science 292:1681.
Entrez Gene IDs	375790 (Human); 11603 (Mouse); 25592 (Rat)
Alternate Names	AGRN, Agrin, agrin proteoglycan

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Recombinant Mouse Agrin His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11573-AG

Key Product Details

Product Specifications for Recombinant Mouse Agrin His-tag Protein, CF

Scientific Data Examples for Recombinant Mouse Agrin His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Agrin

Customer Reviews for Recombinant Mouse Agrin His-tag Protein, CF

Product Documents for Recombinant Mouse Agrin His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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