Recombinant Mouse LRP-4 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10229-LR

R&D Systems, part of Bio-Techne
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10229-LR-025
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Key Product Details

Source

HEK293

Accession #

Q8VI56.3

Conjugate

Unconjugated

Applications

Bioactivity

View all LRP-4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse LRP-4 protein
Mouse LRP-4
(Ser21-Ser1725)
Accession # Q8VI56.3		 HPGGGSGGGSGGGS  HHHHHH
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser21

Predicted Molecular Mass

192 kDa

SDS-PAGE

198-235 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse USAG-1 (Catalog # 9008-SD) is present at 2 μg/mL, the concentration of Recombinat Mouse LRP‑4 that produces 50% of the optimal binding response is 80-800 ng/mL.

Scientific Data Images for Recombinant Mouse LRP-4 His-tag Protein, CF

Recombinant Mouse LRP-4 His-tag Protein Binding Activity

Recombinant Mouse LRP-4 His-tag Protein Binding Activity

When Recombinant Mouse USAG1 (Catalog # 9008-SD) is used at 2 µg/mL (100 µL/well), it binds to Recombinant Mouse LRP-4 (Catalog # 10229-LR) with an ED50 of 80-800 ng/mL.
Recombinant Mouse LRP-4 His-tag Protein SDS-PAGE

Recombinant Mouse LRP-4 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse LRP-4 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 180-235 kDa.

Formulation, Preparation and Storage

10229-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-4

LDL receptor-related protein 4 (LRP-4), also known as Megf7, is an approximately 220 kDa type I membrane protein in the LDL receptor superfamily. LRP-4 plays an important role in development of the neuromuscular junction (NMJ), bones, teeth, mammary placodes, and hair follicles (1, 2). Mature mouse LRP-4 consists of a 1705 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 159 aa cytoplasmic domain. The ECD contains 3 EGF-like domains, 8 LDLR-A domains, and 20 LDLR-B repeats that fold into 4 beta-propeller domains (3, 4). Within the ECD, mouse LRP-4 shares 97% and 99% aa sequence identity with human and rat LRP-4, respectively. The ECD of LRP-4 can be shed by ADAM10 mediated cleavage (5). LRP-4 is expressed on myotubes (6, 7), neurons (8, 9), osteocytes and osteoblasts (10, 11), developing tooth epithelium (12), and male and female germ cells (13). It is localized to NMJ on developing myotubes, and it associates in cis with the muscle-specific kinase MuSK (6, 7). Its binding to neuron-derived Agrin promotes additional LRP-4 association with MuSK, MuSK activation, clustering of AChR, and postsynaptic development of the NMJ (6, 7, and 14). LRP-4 also enhances presynaptic differentiation of the NMJ, although this does not require Agrin (14). Neuronal LRP-4 additionally binds ApoE and Fspondin (8, 9). LRP-4 regulates both BMP and Wnt signaling through binding to Wise, Sclerostin, and Dkk1 (10, 12). These interactions are important for the control of bone growth and mineralization, tooth morphogenesis, and the development of mammary placodes, hair follicles, and whiskers (10, 12, and 15).

References

  1. Zong, Y. and R. Jin (2012) Cell. Mol. Life Sci. Nov 22 Epub.
  2. Ohazama, A. et al. (2010) Am. J. Med. Genet. A 152A:2974.
  3. Nakayama, M. et al. (1998) Genomics 51:27.
  4. Tomita, Y. et al. (1998) J. Biochem. 124:784.
  5. Dietrich, M.F. et al. (2010) PLoS ONE 5:e9960.
  6. Kim, N. et al. (2008) Cell 135:334.
  7. Zhang, B. et al. (2008) Neuron 60:285.
  8. Lu, Y. et al. (2007) Brain Res. 1177:19.
  9. Zisman, S. et al. (2007) J. Cell Biol. 178:1237.
  10. Leupin, O. et al. (2011) J. Biol. Chem. 286:19489.
  11. Choi, H.Y. et al. (2009) PLoS ONE 4:e7930.
  12. Ohazama, A. et al. (2008) PLoS ONE 3:e4092.
  13. Yamaguchi, Y.L. et al. (2006) Gene Expr. Patterns 6:607.
  14. Yumoto, N. et al. (2012) Nature 489:438.
  15. Ahn, Y. et al. (2013) Development 140:583.

Long Name

LDL Receptor-related Protein 4

Alternate Names

LRP10, LRP4

Entrez Gene IDs

4038 (Human); 228357 (Mouse); 83469 (Rat)

Gene Symbol

LRP4

UniProt

Q8VI56.3

Additional LRP-4 Products

Product Documents for Recombinant Mouse LRP-4 His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse LRP-4 His-tag Protein, CF

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