Recombinant Mouse Ameloblastin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3026-AB

R&D Systems, part of Bio-Techne
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3026-AB-050
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Key Product Details

Source

NS0

Accession #

AAH87927

Conjugate

Unconjugated

Applications

Bioactivity

View all Ameloblastin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Ameloblastin protein
Val27-Pro422, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val27 & Ala29

Predicted Molecular Mass

43 kDa

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 1-4 μg/mL.

Formulation, Preparation and Storage

3026-AB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ameloblastin

Ameloblastin (AMBN) is a secreted bone and tooth-specific glycoprotein member of the enamel matrix protein family. Mouse Ameloblastin is synthesized by ameloblasts and osteoblasts as a 407 amino acid (aa; short or “a” isoform) or 422 aa (long or “b” isoform) protein with predicted molecular masses of 44 kDa or 45 kDa, respectively (1). The long isoform includes an additional 15 aa insert following aa 103 and contains additional glycosylation sites (2). This recombinant protein corresponds to the mature long isoform of mouse Ameloblastin. Ameloblastin may be cleaved following secretion leading to the generation of multiple sized proteins from 17-66 kDa (3-6). Mouse Ameloblastin (long isoform) shares 60% and 96% aa sequence identity with the human and rat orthologs, respectively. Ameloblastin is a critical component of tooth enamel and bone matrix where it binds calcium, thereby facilitating bone and tooth mineralization. It has also been shown to self-associate into ribbon-like supramolecular structures (7). During tooth development, it acts by binding to and maintaining the differentiated phenotype of secretory ameloblasts (8, 9). During bone development, Ameloblastin regulates osteogenic differentiation by binding to CD63 and promoting CD63-Integrin beta 1 interactions (10). Ameloblastin may also promote osteoclastogenesis (11). Ameloblastin can act as an adhesion molecule and promotes the adhesion of periodontal ligament cells via RhoA activation and ameloblasts via heparin-binding domains (8, 12, 13).

References

  1. Simmons, D. et al. (1998) Connect Tissue Res. 39:3.
  2. Kobayashi, K. et al. (2007) J. Dent. Res. 86:962.
  3. Uchida, T. et al. (1997) J. Histochem. Cytochem. 45:1329.
  4. Chun, Y.H. et al. (2010) J. Dent. Res. 89:785.
  5. Ravindranath, R.M. et al. (2007) J. Biol. Chem. 282:36370.
  6. Yamakoshi, Y. et al. (2011) Eur. J. Oral Sci. 119 Suppl 1:206.
  7. Wald, T. et al. (2013) J. Biol. Chem. 288:22333.
  8. Fukumoto, S. et al. (2004) J. Cell Biol. 167:973.
  9. Fukumoto, S. et al. (2005) Cells Tissues Organs 181:189.
  10. Iizuka, S. et al. (2011) Mol. Cell Biol. 31:783.
  11. Lu, X. et al. (2013) Bone 54:157.
  12. Zhang, Y. et al. (2011) Eur. J. Oral. Sci. 119:Suppl 1:280.
  13. Sonoda, A. et al. (2009) J. Biol. Chem. 284:27176.

Alternate Names

AMBN

Entrez Gene IDs

258 (Human); 11698 (Mouse)

Gene Symbol

AMBN

UniProt

AAH87927

Additional Ameloblastin Products

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