Recombinant Mouse AMIGO2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9424-AM

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9424-AM-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q80ZD9

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all AMIGO2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse AMIGO2 protein
Mouse AMIGO2
(Gly38-His392)
Accession # Q80ZD9		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly38

Predicted Molecular Mass

67 kDa

SDS-PAGE

80-92 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3.
The ED50 for this effect is 0.3-1.5 μg/mL.

Scientific Data Images for Recombinant Mouse AMIGO2 Fc Chimera Protein, CF

Recombinant Mouse AMIGO2 Fc Chimera Protein Bioactivity

Recombinant Mouse AMIGO2 Fc Chimera Protein Bioactivity

Recombinant Mouse AMIGO2 Fc Chimera (Catalog # 9424-AM) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 0.3-1.5 μg/mL.

Formulation, Preparation and Storage

9424-AM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMIGO2

AMIGO2 (amphoterin‑induced gene and ORF 2), also known as DEGA and Alivin‑1, is an approximately 65 kDa transmembrane cell adhesion protein. It belongs to a family of leucine‑rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1, 2). Mature mouse AMIGO2 consists of a 359 amino acid (aa) extracellular domain (ECD) with six LRRs flanked by single LRRNT and LRRCT domains, followed by one immunoglobulin-like domain, a 21 aa transmembrane segment, and a 101 aa cytoplasmic domain (3-5). Within the ECD, mouse AMIGO2 shares 89% and 94% aa sequence identity with human and rat AMIGO2, respectively. AMIGO2 forms homodimers as well as heterodimers with the related AMIGO1 and AMIGO3 molecules (3, 6). Within the nervous system, AMIGO2 is transcribed in the cerebrum, hypothalamus, olfactory bulb, retina, hippocampus (pyramidal cells), and cerebellum (granule neurons and Purkinje cells) (3, 4, 7). It is also expressed in the liver, lung, testis, spleen, and small intestine (3, 4). AMIGO2 supports neuron survival, and is down-regulated following pro‑apoptotic stimulation (4). Its expression can be up‑regulated or down-regulated in a variety of cancers, and anti‑sense knockdown of AMIGO2 interferes with tumor cell adhesion to collagen as well as in vivo tumorigenicity (5). AMIGO2 modulates T cell functions and its deficiency in mice ameliorates experimental autoimmune encephalomyelitis (6).

References

  1.  de Wit, J. et al. (2011) Annu. Rev. Cell Dev. Biol. 27:697.
  2. Chen, Y. et al. (2006) Brain Res. Rev. 51:265.
  3. Kuja-Panula, J. et al. (2003) J. Cell Biol. 160:963.
  4. Ono, T. et al. (2003) J. Neurosci. 23:5887.
  5. Rabenau, K.E. et al. (2004) Oncogene 23:056.
  6. Li, Z., et al. (2017) Brain Behav. Immun. 62:110.

Long Name

Amphoterin-induced Gene and ORF 2

Alternate Names

Alivin-1

Entrez Gene IDs

347902 (Human); 105827 (Mouse); 102124161 (Cynomolgus Monkey)

Gene Symbol

AMIGO2

UniProt

Q80ZD9

Additional AMIGO2 Products

Product Documents for Recombinant Mouse AMIGO2 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse AMIGO2 Fc Chimera Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.