Recombinant Mouse ASIP/Agouti Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9619-AG

R&D Systems, part of Bio-Techne
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9619-AG-050
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Key Product Details

Source

NS0

Accession #

Q03288

Conjugate

Unconjugated

Applications

Bioactivity

View all ASIP Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse ASIP protein
His23-Cys131

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His23

Predicted Molecular Mass

12 kDa

SDS-PAGE

19-24 kDa, reducing conditions

Activity

Measured by its ability to inhibit alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells.
The ED50 for this effect is 0.8-4 μg/mL.

Scientific Data Images for Recombinant Mouse ASIP/Agouti Protein, CF

Recombinant Mouse ASIP/Agouti Protein Bioactivity

Recombinant Mouse ASIP/Agouti Protein Bioactivity

Recombinant Mouse ASIP/Agouti (Catalog # 9619-AG) inhibits alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED50 for this effect is 0.8-4 μg/mL.

Formulation, Preparation and Storage

9619-AG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASIP

Agouti, also known as Agouti Signaling Protein (ASIP), is a 131 amino peptide that regulates hair pigmentation in mice and adipocyte metabolism in humans (1). Mature mouse Agouti shares 78% and 94% amino acid sequence identity with human and rat Agouti, respectively (2, 3). Human Agouti is primarily expressed in adipose tissue, whereas its adipose expression in mice is only induced by mutations in the Agouti promoter (4, 5). Studies of human Agouti have shown that it is also expressed in the pancreas where it enhances the production of insulin by beta cells (6). In humans and mice, Agouti functions as a competitive antagonist at several melanocortin receptors. It blocks ACTH binding to MC1R and MSH binding to MC1R, MC3R, and MC4R (7-9). It blocks MSH induced effects on melanocytes including proliferation and melanin production (3, 7, 8).

References

  1. Voisey, J. and A. van Daal (2002) Pigment Cell Res. 15:10.
  2. Kwon, H.Y. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9760.
  3. Wilson, B.D. et al. (1995) Hum. Mol. Genet. 4:223.
  4. Mynatt, R.L. et al. (1997) Proc. Natl. Acad. Sci. USA 94:919.
  5. Mynatt, R.L. and J.M. Stephens (2001) Am. J. Physiol. Cell Physiol. 280:C954.
  6. Xue, B.Z. et al. (1999) Physiol. Genomics 1:11.
  7. Lu, D. et al. (1994) Nature 371:799.
  8. Suzuki, I. et al. (1997) J. Invest. Dermatol. 108:838.
  9. Kiefer, L.L. et al. (1997) Biochemistry 36:2084.

Long Name

Agouti Signaling Protein

Alternate Names

AGSW, AGTI, AGTIL, SHEP9

Entrez Gene IDs

434 (Human); 50518 (Mouse); 24152 (Rat)

Gene Symbol

ASIP

UniProt

Q03288

Additional ASIP Products

Product Documents for Recombinant Mouse ASIP/Agouti Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse ASIP/Agouti Protein, CF

For research use only

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