Recombinant Mouse B7-1/CD80 His tagged Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9014-B1

R&D Systems, part of Bio-Techne
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9014-B1-01M
9014-B1-200
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Key Product Details

Source

NS0

Accession #

Q00609

Conjugate

Unconjugated

Applications

Bioactivity

View all B7-1/CD80 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse B7-1/CD80 protein
Asp37-Lys245, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp37

Predicted Molecular Mass

25 kDa

SDS-PAGE

39-54 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CTLA-4 Fc Chimera (Catalog # 434-CT) is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Mouse B7-1/CD80 that produces 50% of the optimal binding response is approximately 5-30 ng/mL.

Formulation, Preparation and Storage

9014-B1
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-1/CD80

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-6). Mature mouse B7-1 consists of a 209 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 22 aa transmembrane segment, and a 38 aa cytoplasmic domain (7). Within the ECD, mouse B7-1 shares 50% and 68% aa sequence identity with human and rat B7-1, respectively. Alternative splicing generates a short isoform that lacks the second Ig-like domain but retains the ability to bind CD28 (8). Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4 (1). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2). B7-1 and B7-2 interact with CD28 to modulate short- and long-lived plasma cell functions.

References

  1. Ville, S. et al. (2015) Front. Immunol. 6:411.
  2. Azuma, M. et al. (1993) Nature 366:76.
  3. Freeman, G.J. et al. (1993) Science 262:909.
  4. Chen, C. et al. (1994) J. Immunol. 152:4929.
  5. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
  6. Lanier, L. et al. (1995) J. Immunol. 154:97.
  7. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
  8. Inobe, M. et al. (1994) Biochem. Biophys. Res. Commun. 200:443.
  9. Njan, MN. et al. (2012) J. Immunol. 189:2758

Alternate Names

B71, CD80

Entrez Gene IDs

941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)

Gene Symbol

CD80

UniProt

Q00609

Additional B7-1/CD80 Products

Product Documents for Recombinant Mouse B7-1/CD80 His tagged Protein, CF

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Product Specific Notices for Recombinant Mouse B7-1/CD80 His tagged Protein, CF

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