Recombinant Mouse BACE-2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2977-AS
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse BACE-2 protein
Phe29-Pro462 (Tyr357His) with a C-terminal 6-His tag
Phe29-Pro462 (Tyr357His) with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Phe29, Ala63, Asp140
Predicted Molecular Mass
48 kDa
SDS-PAGE
55-76 kDa, reducing conditions
Activity
Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog # ES010).
The specific activity is >50 pmol/min/µg, as measured under the described conditions.
The specific activity is >50 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2977-AS
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution |
Reconstitute at 500 μg/mL in sterile, deionized water.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: BACE-2
BACE-2 is an aspartic protease that shares sequence similarity with BACE-1, the major beta‑secretase for generation of A beta peptide in neurons (1). However, BACE-2 differs from BACE-1 in several aspects including pro-enzyme activation, substrate binding sites, transcriptional regulation, and function in A beta peptide production (2‑4). The domain structure of mouse BACE-2 consists of a signal peptide (aa 1‑19), pro (aa 20‑62) and a mature chain (aa 63‑514) that consists of a transmembrane domain and a cytoplasmic tail. The C‑terminal region (aa 463‑514) was replaced by a His tag in the Recombinant Mouse BACE-2 that also contained a His residue at the position of Tyr357. The purified rmBACE-2 started at Ala63, the same residue shown at the N-terminus of the human active BACE-2 (2).
References
- Cai, H. et al. (2001) Nature Neurosci. 4:233.
- Hussain, I. et al. (2001) J. Biol. Chem. 276:23322.
- Ostermann, N. et al. (2006) J. Mol. Biol. 355:249.
- Sun, X. et al. (2005) FASEB J. 19:739.
Long Name
beta-site Ayloid Precursor Protein Cleaving Enzyme 2
Alternate Names
BACE2
Gene Symbol
BACE2
UniProt
Additional BACE-2 Products
Product Documents for Recombinant Mouse BACE-2 Protein, CF
Product Specific Notices for Recombinant Mouse BACE-2 Protein, CF
For research use only
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