Recombinant Mouse BAMBI/NMA Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8359-BM

R&D Systems, part of Bio-Techne
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8359-BM-050
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Key Product Details

Source

NS0

Accession #

Q9D0L6

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BAMBI/NMA Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse BAMBI/NMA protein
Mouse BAMBI/NMA
(Glu27-Ala152)
Accession # Q9D0L6		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu27

Predicted Molecular Mass

41 kDa

SDS-PAGE

48-53 kDa, reducing conditions

Activity

Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells.
The ED50 for this effect is 1-6 μg/mL.

Formulation, Preparation and Storage

8359-BM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAMBI/NMA

BMP and Activin Membrane-Bound Inhibitor (BAMBI), also called Non-Metastatic Gene A (NMA), is a type I transmembrane protein that shares sequence homology with the TGF-beta type I receptor family and functions as a decoy receptor (1). Mouse BAMBI is synthesized as a 260 amino acid (aa) precursor and has a predicted molecular weight of approximately 29 kDa (1). Its extracellular domain contains only one potential N-glycosylation site, and shares 91% and 98% aa sequence identity with the human and rat orthologs, respectively. BAMBI differs from other members of the TGF-beta RI family in that it has a short cytoplasmic region that lacks the intracellular serine/threonine kinase domain (1, 2). It competes with type I receptors to form heterodimers with type II receptors, thus interfering with BMP, Activin, and TGF-beta signaling (2). In fact, BAMBI is believed to be one component of a negative feedback loop for BMP signaling that serves to increase the dynamic signaling range for BMPs (3, 4). During development, BAMBI is prominently expressed in gastrulation, neurulation, and during the development of teeth and bones, and is often co-expressed with other BMP family members (1-5). BAMBI is also ubiquitously expressed in the adult, with the highest levels being observed in heart, lung, spleen, kidney, bladder, and testes (6). BAMBI expression is induced by TGF-beta and Wnt signaling (7, 8). It cooperates with inhibitory Smad6 and Smad7 to inhibit TGF-beta signaling (9). In contrast, BAMBI promotes Wnt signaling via its interactions with Frizzled receptors (10). BAMBI has been shown to modulate various processes including adipogenesis, thrombus formation and stability, cell proliferation, and opioid signaling in neuropathic pain (10-13). Additionally, aberrant BAMBI expression is believed to be a factor in the development of cancer, inflammation, and fibrotic processes (7, 14-18).

References

  1. Knight, C. et al. (2001) J. Dent. Res. 80:1895.
  2. Onichtchouk, D. et al. (1999) Nature 401:480.
  3. Paulsen, M. et al. (2011) Proc. Natl. Acad. Sci. USA 108:10202.
  4. Montero, J.A. et al. (2008) Dev. Biol. 321:343.
  5. Grotewold, L. et al. (2001) Mech. Dev. 100:327.
  6. Lakoski, K. et al. (2003) Endocrinology 144:4180.
  7. Sekiya, T. et al. (2004) J. Biol. Chem. 279:6840.
  8. Sekiya, T. et al. (2004) Biochem. Biophys. Res. Commun. 320:680.
  9. Yan, X. et al. (2009) J. Biol. Chem. 284:30097.
  10. Lin, Z. et al. (2008) J. Biol. Chem. 283:33053.
  11. Luo, X. et al. (2012) Diabetes 61:124.
  12. Salles-Crawley, I.I. et al. (2014) Blood 123:2873.
  13. Lantero, A. et al. (2014) J. Neurosci. 34:5385.
  14. Seki, E. et al. (2007) Nat. Med. 13:1324.
  15. Khin, S.S. et al. (2009) Int. J. Cancer 125:328.
  16. Fritzmann, J. et al. (2009) Gastroenterology 137:165.
  17. Drömann, D. et al. (2010) Respir. Res. 11:67.
  18. Li, Y.S. et al. (2013) PLoS One 8:e76289.

Long Name

BMP and Activin Membrane-bound Inhibitor

Alternate Names

NMA

Entrez Gene IDs

25805 (Human); 68010 (Mouse)

Gene Symbol

BAMBI

UniProt

Q9D0L6

Additional BAMBI/NMA Products

Product Documents for Recombinant Mouse BAMBI/NMA Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse BAMBI/NMA Fc Chimera Protein, CF

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