Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 10336-BL
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse BLAME/SLAMF8 protein
Mouse BLAME/SLAMF8 (Val21-Asp231) Accession # NP_083360.2 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val21
Predicted Molecular Mass
50 kDa
SDS-PAGE
52-70 kDa, under reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein (Catalog # 10336-BL) is immobilized at 5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein that produces 50% of the optimal binding response is found to be approximately 4‑20 µg/mL.
When Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein (Catalog # 10336-BL) is immobilized at 5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein that produces 50% of the optimal binding response is found to be approximately 4‑20 µg/mL.
Scientific Data Images for Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein, CF
Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein Binding Activity
When Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein (Catalog # 10336-BL) is immobilized at 5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein that produces 50% of the optimal binding response is found to be approximately 4-20 μg/mL.Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein SDS-PAGE
2 μg/lane of Recombinant Mouse BLAME/SLAMF8 Fc Chimera (Catalog # 10336-BL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-70 kDa and 100-140 kDa, respectively.Formulation, Preparation and Storage
10336-BL
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution | Reconstitute at 200 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: BLAME/SLAMF8
References
- McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91.
- Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
- Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.
- Wang, G. et al. (2012) J. Immunol. 188:5829.
Long Name
SLAM Family Member 8
Alternate Names
CD353, SBBI42, SLAMF8
Gene Symbol
SLAMF8
UniProt
Additional BLAME/SLAMF8 Products
Product Documents for Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein, CF
For research use only
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