Recombinant Mouse BMP-8a Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7540-BP

R&D Systems, part of Bio-Techne
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7540-BP-025
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Key Product Details

Source

CHO

Accession #

P34821

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BMP-8a Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse BMP-8a protein
Ala261-His399

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala261

Predicted Molecular Mass

15.7 kDa (monomer)

SDS-PAGE

19 kDa, reducing conditions

Activity

Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. Miyazaki, H. et al. (2004) Oncogene 23:9326.
The ED50 for this effect is 0.5-5 μg/mL.

Formulation, Preparation and Storage

7540-BP
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution
Reconstitute at 250 μg/mL in 4 mM HCl.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-8a

BMP‑8, also known as osteogenic protein 2 (OP‑2), was first isolated from a hippocampal library in a screen to identify relatives of BMP‑7/OP‑1 (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF‑ beta) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero‑oligomeric complexes of type I and type II serine/threonine kinase receptors (2‑4). BMP‑8a and BMP‑8b, produced from separate genes, share 98% amino acid sequence identity in human but only 74% in mouse within the mature regions. Mouse BMP‑8a is synthesized as a 399 aa precursor protein that is cleaved between Arg260 and Ala261 to release the C‑terminal mature protein. Mature mouse BMP‑8a shares 90% aa sequence identity with mature human BMP‑8a and ‑8b. BMP‑8a is expressed during pregnancy in the deciduum and trophoblast cells, by inner root sheath cells of developing hair follicles, and by the epididymis and spermatids (5‑7). In the mouse it cooperates with BMP‑7 in the maintenance of spermatogenesis but is not required for the initiation of spermatogenesis (7, 8). BMP‑8b, in contrast, is required for both the initiation and maintenance of spermatogenesis (9). BMP‑8a is induced during osteoblast differentiation at the onset of mineralization and during the osteogenic phase of bone repair in osteoblasts and osteocytes (10‑12). BMP‑8a/b is also highly expressed in osteosarcomas (13).

References

  1. Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
  2. Chen, D. et al. (2004) Growth Factors 22:233.
  3. Bragdon, B. et al. (2010) Cell Signal. 23:609.
  4. Singh, A. and R.J. Morris (2010) Cytokine Growth Factor Rev. 21:299.
  5. Zhao, G.-Q. and B.L. Hogan (1996) Mech. Dev. 57:159.
  6. Ying, Y. and G.-Q. Zhao (2000) Biol. Reprod. 63:1781.
  7. Zhao, G.-Q. et al. (1998) Development 125:1103.
  8. Zhao, G.-Q. et al. (2001) Dev. Biol. 240:212.
  9. Zhao, G.-Q. et al. (1998) Genes Dev. 10:1657.
  10. van der Horst, G. et al. (2002) Bone 31:661.
  11. Cho, T.-J. et al. (2002) J. Bone Miner. Res. 17:513.
  12. Paic, F. et al. (2009) Bone 45:682.
  13. Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.

Long Name

Bone Morphogenetic Protein 8a

Alternate Names

Op2, Osteogenic Protein 2

Entrez Gene IDs

353500 (Human); 12163 (Mouse)

Gene Symbol

BMP8A

UniProt

P34821

Additional BMP-8a Products

Product Documents for Recombinant Mouse BMP-8a Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse BMP-8a Protein, CF

For research use only

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