Recombinant Mouse BMPR-IA/ALK-3 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7054-MR

R&D Systems, part of Bio-Techne
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7054-MR-100
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Key Product Details

Source

NS0

Accession #

NP_033888

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BMPR-IA/ALK-3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse BMPR-IA/ALK-3 protein
Mouse BMPR-1A/ALK-3
(Met1 - Arg152)
Accession # NP_033888		 IEGRDP MouseIgG2A
(Glu98 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln24 predicted, N-sequencing might be blocked

Predicted Molecular Mass

41.3 kDa (monomer)

SDS-PAGE

57-60 kDa, reducing conditions

Activity

Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.

The ED50 for this effect is 0.03-0.12 μg/mL in the presence of 30 ng/mL of recombinant human BMP-4.

Formulation, Preparation and Storage

7054-MR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMPR-IA/ALK-3

Bone Morphogenetic Protein Receptor IA (BMPR‑IA), also known as ALK‑3, BRK‑1, and CD292, is a glycosylated 60 ‑ 65 kDa type I receptor in the TGF‑ beta serine/threonine kinase receptor family (1 ‑ 3). Binding of TGF‑ beta superfamily ligands induces formation of a heterotetrameric complex that contains two chains each of a type I and a type II receptor in multiple combinations. The type II receptors phosphorylate the type I receptors which then phosphorylate and activate Smad signal transduction proteins (1, 2). Mature mouse BMPR‑IA consisits of a 129 amino acid (aa) extracellular domain (ECD), a 24 aa transmembrane segment, and a 356 aa cytoplasmic region that contains the tyrosine kinase domain (4, 5). Within the ECD, mouse BMPR‑IA shares 98% aa sequence identity with human and rat BMPR‑IA. BMPR‑IA is involved in the development and function of a wide range of tissues. During early embryogenesis it is required for migration of the anterior visceral endoderm (AVE) and proper development of the anterior‑posterior axis (6). Tissue‑specific conditional knockout experiments have demonstrated the importance of BMPR‑IA in the development and morphogenesis of the heart, lung, palate, teeth, and mandible (7 ‑ 9). In the adult, BMPR‑IA plays a role in glucose-stimulated insulin secretion by pancreatic beta cells, osteoclast activity and bone remodeling, reactive astrocyte‑mediated scar formation following spinal cord injury, and ovulation and fertility (10 ‑ 13).

References

  1. Wu, M.Y. and C.S. Hill (2009) Dev. Cell 16:329.
  2. Nickel, J. et al. (2009) Cytokine Growth Factor Rev. 20:367.
  3. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  4. Dewulf, N. et al. (1995) Endocrinology 136:2652.
  5. Koenig, B.B. et al. (1994) Mol. Cell. Biol. 14:5961.
  6. Miura, S. et al. (2010) Dev. Biol. 341:246.
  7. Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878.
  8. Sun, J. et al. (2008) Am. J. Pathol. 172:571.
  9. Li, L. et al. (2011) Dev. Biol. 349:451.
  10. Goulley, J. et al. (2007) Cell Metab. 5:207.
  11. Kamiya, N. et al. (2008) J. Bone Miner. Res. 23:2007.
  12. Sahni, V. et al. (2010) J. Neurosci. 30:1839.
  13. Edson, M.A. et al. (2010) Mol. Endocrinol. 24:1251.

Long Name

Bone Morphogenetic Protein Receptor IA/Activin Receptor-like Kinase 3

Alternate Names

ALK-3, BMPR1A, BMPRIA, CD292

Entrez Gene IDs

657 (Human); 12166 (Mouse)

Gene Symbol

BMPR1A

UniProt

NP_033888

Additional BMPR-IA/ALK-3 Products

Product Documents for Recombinant Mouse BMPR-IA/ALK-3 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse BMPR-IA/ALK-3 Fc Chimera Protein, CF

For research use only

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