Recombinant Mouse Brevican Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7188-BC

R&D Systems, part of Bio-Techne
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7188-BC-050
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Key Product Details

Source

NS0

Accession #

Q61361

Conjugate

Unconjugated

Applications

Bioactivity

View all Brevican Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Brevican protein
Met1-Leu883, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp23

Predicted Molecular Mass

94.5 kDa

SDS-PAGE

130-145 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Mouse Brevican is coated at 3 μg/mL (100 μL/well), the concentration of biotinylated hyaluronan that produces 50% of the optimal binding response is found to be approximately 1-6 ng/mL.

Formulation, Preparation and Storage

7188-BC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Brevican

Brevican, also called BEHAB, is a member of the the lectican family of proteoglycans that share a common domain structure (1). It is restricted to the CNS and associates with perineuronal nets and astrocytes (2 ‑ 5). Brevican contains an Ig-like V-set domain, two link domains, a Glu-rich region, a central region with glycosaminoglycan (GAG) modifications, an EGF-like domain, a C-type lectin domain, and a C-terminal Sushi/CRP-like domain (6). Brevican consists of a 145 kDa core protein with up to approximately 100 kDa of attached chondroitin sulfate but not heparan sulfate chains (3, 7). Alternate splicing generates a GPI-anchored isoform that lacks the EGF-like, lectin, and Sushi domains (4, 6). Brevican is cleaved by multiple proteases and exists in a number of distinct fragments (8, 9). An under-glycosylated form of Brevican accumulates in highly aggressive glioma, Alzheimer’s-like brain, and following brain injury (10 - 12). Levels of an ADAMTS-generated 55 kDa N-terminal fragment accumulate during remodeling after excitotoxic injury, and Brevican cleavage is required for its ability to promote glioma adhesion and motility (13 ‑ 15). In contrast, Brevican cleavage is inhibited in mouse brain with amyloid beta deposits (10). Mouse Brevican shares 80%, 81%, and 95% aa sequence identity with bovine, human, and rat Brevican, respectively. Within the Ig-like and two link domains, mouse Brevican shares 47% - 51% aa sequence identity with mouse Aggrecan, Neurocan, and Versican.

References

  1. Maeda, N. et al. (2010) Front. Biosci. 15:626.
  2. Jaworski, D.M. et al. (1994) J. Cell Biol. 125:495.
  3. Yamada, H. et al. (1997) J. Neurosci. 17:7784.
  4. Seidenbecher, C.I. et al. (2002) J. Neurochem. 83:738.
  5. Deepa, S.S. et al. (2006) J. Biol. Chem. 281:17789.
  6. Rauch, U. et al. (1997) Genomics 44:15.
  7. Seidenbecher, C.I. et al. (1995) J. Biol. Chem. 270:27206.
  8. Matthews, R.T. et al. (2000) J. Biol. Chem. 275:22695.
  9. Nakamura, H. et al. (2000) J. Biol. Chem. 275:38885.
  10. Ajmo, J.M. et al. (2010) J. Neurochem. 113:784.
  11. Viapiano, M.S. et al. (2005) Canc. Res. 65:6726.
  12. Viapiano, M.S. et al. (2003) J. Biol. Chem. 278:33239.
  13. Hu, B. et al. (2008) J. Biol. Chem. 283:24848.
  14. Mayer, J. et al. (2005) BMC Neurosci. 6:52.
  15. Yuan, W. et al. (2002) Neuroscience 114:1091.

Long Name

Brain Specific Proteoglycan in the Aggrecan Family

Alternate Names

ALPBRE, BCAN, BEHAB, CSPG7

Entrez Gene IDs

63827 (Human); 12032 (Mouse); 25393 (Rat)

Gene Symbol

BCAN

UniProt

Q61361

Additional Brevican Products

Product Documents for Recombinant Mouse Brevican Protein, CF

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Product Specific Notices for Recombinant Mouse Brevican Protein, CF

For research use only

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