Recombinant Mouse BTLA Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3007-BT

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q32MV9

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all BTLA Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse BTLA protein
Mouse BTLA
Glu30-Gly176 (Lys173Glu)
Accession # Q32MV9		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu30

Predicted Molecular Mass

44 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured in a competitive binding assay.
When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Mouse BTLA Fc Chimera inhibits 50% binding of biotinylated Recombinant Mouse BTLA Fc Chimera (0.2 µg/mL) at the concentration range of 0.3-1 µg/mL.

Scientific Data Images for Recombinant Mouse BTLA Fc Chimera Protein, CF

Recombinant Mouse BTLA Fc Chimera Protein Binding Activity

Recombinant Mouse BTLA Fc Chimera Protein Binding Activity

When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is coated at 0.1 µg/mL, Recombinant Mouse BTLA Fc Chimera inhibits the binding of biotinylated Recombinant Mouse BTLA Fc Chimera with an IC50of 0.3-1 µg/mL

Formulation, Preparation and Storage

3007-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTLA

B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell co-stimulatory molecules (1-3). Mature mouse BTLA contains a 154 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane sequence, and a 102 aa cytoplasmic domain. The two ITIM motifs and three Tyr phosphorylation sites in the cytoplasmic tail transmit inhibitory signaling (4-5). The ECD of mouse BTLA shares 51% and 77% aa identity with that of human and rat BTLA, respectively. A splice variant lacking the transmembrane domain has been reported (6). Unlike other CD28 family members, the BTLA Ig domain in the ECD is of the I-type rather than V-type, and BTLA does not form homodimers (7). BTLA is also unusual in its interaction with the TNF superfamily member HVEM rather than with B7 family ligands (8). BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and NK cells (9). Its expression is low in naïve T cells and increases during antigen-specific induction of anergy. In B cells, BTLA expression is highest in mature naïve cells (9). BTLA apparently limits T cell numbers, since its deletion results in over-production of T cells, especially CD8+ memory T cells that are hyper-responsive to TCR cross-linking (10). Under the control of ROR gammat and IL-7, BTLA regulates the homeostasis and inflammatory responses of gamma deltaT cells (11). The binding of BTLA and HVEM does not preclude the concurrent binding of other HVEM ligands such as LIGHT or Lymphotoxin-alpha (12).

References

  1. Murphy, K.M. et al. (2006) Nat. Rev. Immunol. 6:671.
  2. Croft, M. (2005) Trends. Immunol. 26:292.
  3. Watanabe, N. et al. (2003) Nat. Immunol. 4:670.
  4. Gavrieli, M. et al. (2003) Biochem. Biophys. Res. Commun. 312:1236.
  5. Chemnitz, J.M. et al. (2006) J. Immunol. 176:6603.
  6. Han, P. et al. (2004) J. Immunol. 172 :5931.
  7. Compaan, D.M. et al. (2005) J. Biol. Chem. 280:39553.
  8. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
  9. Hurchla, M.A. et al. (2005) J. Immunol. 174:3377.
  10. Krieg, C. et al. (2007) Nat. Immunol. 8:162.
  11. Bekiaris, V. et al. (2013) Immunity 39:1082.
  12. Cai G and Freeman GJ, (2009) Immunol Rev. 229:244

Long Name

B- And T-Lymphocyte Attenuator

Alternate Names

CD272

Entrez Gene IDs

151888 (Human); 208154 (Mouse); 102144877 (Cynomolgus Monkey)

Gene Symbol

BTLA

UniProt

Q32MV9

Additional BTLA Products

Product Documents for Recombinant Mouse BTLA Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse BTLA Fc Chimera Protein, CF

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