Recombinant Mouse BTNL9 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9555-BT

R&D Systems, part of Bio-Techne
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9555-BT-050
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Key Product Details

Source

NS0

Accession #

Q8BJE2-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BTNL9 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse BTNL9 protein
Mouse BTNL9
(Asp36-Lys257)
Accession # Q8BJE2-1		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp36

Predicted Molecular Mass

52 kDa

SDS-PAGE

60-73 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3.
The ED50 for this effect is 0.75-4.5 μg/mL.

Scientific Data Images for Recombinant Mouse BTNL9 Fc Chimera Protein, CF

Recombinant Mouse BTNL9 Fc Chimera Protein Bioactivity

Recombinant Mouse BTNL9 Fc Chimera Protein Bioactivity

Recombinant Mouse BTNL9 Fc Chimera (Catalog # 9555-BT) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD antibody. The ED50 for this effect is 0.75-4.5 μg/mL.

Formulation, Preparation and Storage

9555-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTNL9

Butyrophilin-like 9 (BTNL9) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Mouse BTNL9 is a 536 amino acid (aa) type I transmembrane glycoprotein that contains a 35 aa signal sequence, a 222 aa extracellular domain (ECD), a 21 aa transmembrane region and a 258 aa cytoplasmic segment. The ECD features two V-type Ig-like domains and three glycosylation sites (2-4). The ECD of mouse BTNL9 shares ~67% and 91% aa sequence identity with the ECD of human and rat BTNL9, respectively. BTNL9 is expressed in a variety of tissues in humans and mice. Among isolated immune cell types, B cells show the strongest expression (2, 5). Recombinant BTNL9–Fc has been shown to bind to many immune cells including T cells, B cells, macrophages and dendritic cells (6). With this profile of binding, it will not be surprising if BTNL9 joined the panel of butyrophilins with negative regulatory effects on immune cells (5). BTNL9 has been reported to inhibit CD8+ T Cell proliferation (5).

References

  1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523
  2. Arnett, H.A. et al. (2009) Cytokine 46:370.
  3. Chapoval, A.I. et al. (2013) Mol. Immunol. 56:819.
  4. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  5. Arnett, H.A. et al. (2014) Nat Rev Immunol. 14:5596.
  6. Yamazaki, T. et al. (2010) J. Immunol. 185:5907.

Long Name

Butyrophilin-like 9

Alternate Names

BTN3, VDLS1900

Entrez Gene IDs

153579 (Human); 237754 (Mouse); 686977 (Rat)

Gene Symbol

BTNL9

UniProt

Q8BJE2-1

Additional BTNL9 Products

Product Documents for Recombinant Mouse BTNL9 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse BTNL9 Fc Chimera Protein, CF

For research use only

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