Recombinant Mouse Carboxypeptidase A4/CPA4 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2899-ZN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Carboxypeptidase A4/CPA4 protein
Gly17-Tyr420, with a C-terminal 10-His tag
Accession # Q6P8K8
Gly17-Tyr420, with a C-terminal 10-His tag
Accession # Q6P8K8
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly17
Predicted Molecular Mass
47 kDa
SDS-PAGE
48 kDa, reducing conditions
Activity
Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >400 pmol/min/µg, as measured under the described conditions.
The specific activity is >400 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2899-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Carboxypeptidase A4/CPA4
Carboxypeptidase A4 encoded by the CPA4 gene is a member of the metallocarobxypeptidase family that cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The deduced amino acid sequence of mouse CPA4 consists of a signal peptide (residues 1 to 16), a pro region (residue 17 to 113), and a mature chain (residues 114 to 420). The purified recombinant mouse CPA4 corresponds mainly to the pro form, which can be activated and assayed under the conditions described in the Activity Assay Protocol.
Additional Carboxypeptidase A4/CPA4 Products
Product Documents for Recombinant Mouse Carboxypeptidase A4/CPA4 Protein, CF
Product Specific Notices for Recombinant Mouse Carboxypeptidase A4/CPA4 Protein, CF
For research use only
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