Recombinant Mouse Carboxypeptidase B1/CPB1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2898-ZN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Carboxypeptidase B1/CPB1 protein
Ser16-Tyr415, with a C-terminal 10-His tag
Accession # NP_083982
Ser16-Tyr415, with a C-terminal 10-His tag
Accession # NP_083982
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser16
Predicted Molecular Mass
47 kDa
SDS-PAGE
43 kDa and 48 kDa, reducing conditions
Activity
Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg.
The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2898-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Carboxypeptidase B1/CPB1
Carboxypeptidase B1, encoded by the CPB1 gene, specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg (1). The deduced amino acid sequence of mouse CPB1 consists of a signal peptide (residues 1 to 15), a pro region (residue 16 to 108), and a mature chain (residues 109 to 415). The purified rmCPB1 corresponds to the pro form, which can be activated by trypsin, the only pancreatic protease capable of generating active enzyme from the zymogen in vitro (1).
References
- Aviles, F.X. and J. Vendrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 831, Academic Press, San Diego.
Alternate Names
CPB1, PASP, PCPB
Gene Symbol
CPB1
UniProt
Additional Carboxypeptidase B1/CPB1 Products
Product Documents for Recombinant Mouse Carboxypeptidase B1/CPB1 Protein, CF
Product Specific Notices for Recombinant Mouse Carboxypeptidase B1/CPB1 Protein, CF
For research use only
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