Recombinant Mouse Caspr2 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10196-CR

R&D Systems, part of Bio-Techne
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10196-CR-100
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Key Product Details

Source

NS0

Accession #

Q9CPW0

Conjugate

Unconjugated

Applications

Bioactivity

View all Caspr2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Caspr2 protein
Ala28-Ser1262, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala28

Predicted Molecular Mass

138 kDa

SDS-PAGE

130-150 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Caspr2 His-tag (Catalog # 10196-CR) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human Contactin-1 Fc Chimera (Catalog # 904-CN) that produces 50% of the optimal binding response is 5-30 μg/mL.

Scientific Data Images for Recombinant Mouse Caspr2 His-tag Protein, CF

Recombinant Mouse Caspr2 His-tag Protein SDS-PAGE

Recombinant Mouse Caspr2 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Caspr2 His-tag (Catalog # 10196-CR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 130-150 kDa.

Formulation, Preparation and Storage

10196-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspr2

Contactin Associated Protein-Like 2 (CASPR2), also known as Cntnap2, is a neuronal type I transmembrane member of the neurexin family of adhesion molecules (ref). CASPR2, along with CASPR1 (or Cntnap1), is required for radical and longitudinal organization of myelinated axons (1). Mature mouse CASPR2 consists of a large extracellular domain (ECD) containing four Laminin G-like domains as well as two EFG-like domains and a Fibrinogen C-terminal domain, a transmembrane segment, and a short cytoplasmic domain. The ECD of mouse CASPR2 shares 94% and 93% amino acid (aa) identity with the human and rat ECD, respectively. CASPR2 is thought to be important for the control of neuron-glia interactions and is associated with alterations in synaptic structure, neuronal network activity, and an autism-related phenotype in mice (2, 3). In myelinated nerves, CASPR2 is confined to the juxtaparanodal region of the axon where it appears to associate with the immunoglobulin domains of TAG-1 (transient axonal glycoprotein 1) to form a scaffold, which clusters the potassium channels Kv1.1 and Kv1.2 (4). Recent studies demonstrate that CASPR2 binds to contactin1 (5).

References

  1. Gordon A. et al. (2014) J. Neurosci. 10:1523.
  2. Cope, E. et al. (2016) eNeuro. 0196-16.
  3. Peñagarikano O. et al. (2011) Cell. 147(1):236.
  4. Poliak, S. et al. (1999) Neuron 24:1037.
  5. Rubio-Marrero, E. N. et al. (2016) J. Bio. Chem. 291:5788.

Long Name

Contactin Associated Protein-like 2

Alternate Names

CDFE, CNTNAP2, NRXN4

Entrez Gene IDs

26047 (Human); 66797 (Mouse)

Gene Symbol

CNTNAP2

UniProt

Q9CPW0

Additional Caspr2 Products

Product Documents for Recombinant Mouse Caspr2 His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse Caspr2 His-tag Protein, CF

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