Recombinant Mouse CCL1/TCA-3 Protein
R&D Systems, part of Bio-Techne | Catalog # 845-TC
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse CCL1/I-309/TCA-3 protein
Lys24-Cys92
Lys24-Cys92
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Lys24
Predicted Molecular Mass
7.8 kDa
SDS-PAGE
13-16 kDa, reducing conditions
Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CCR8.
The ED50 for this effect is 0.5-2.0 ng/mL.
The ED50 for this effect is 0.5-2.0 ng/mL.
Reviewed Applications
Read 2 reviews rated 4.5 using 845-TC in the following applications:
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 845-TC
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 845-TC/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CCL1/I-309/TCA-3
Mouse TCA-3 is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.
TCA-3 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA-3 contains one potential N-linked glycosylation site. Mouse TCA-3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta and JE. TCA-3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA-3 has been shown to chemoattract T-cells.
References
- Goya, I. et al. (1998) J. Immunol. 160:1975.
- Burd, P. et al. (1987) J. Immunol. 139:3126.
Alternate Names
I-309, P500, SCYA1, SCYA2, TCA-3, TCA3
Gene Symbol
CCL1
UniProt
Additional CCL1/I-309/TCA-3 Products
Product Documents for Recombinant Mouse CCL1/TCA-3 Protein
Product Specific Notices for Recombinant Mouse CCL1/TCA-3 Protein
For research use only
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