Recombinant Mouse CCL19/MIP-3 beta Protein
R&D Systems, part of Bio-Techne | Catalog # 440-M3
Key Product Details
Product Specifications
Source
E. coli-derived mouse CCL19/MIP-3 beta protein
Gly26-Val107-Leu-Glu, with a substitution of Ser108LeuGlu
Gly26-Val107-Leu-Glu, with a substitution of Ser108LeuGlu
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly26
Predicted Molecular Mass
9.4 kDa
Activity
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL).
The ED50 for this effect is 0.03-0.1 µg/mL.
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR7.
The ED50 for this effect is 3-15 ng/mL.
The ED50 for this effect is 0.03-0.1 µg/mL.
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR7.
The ED50 for this effect is 3-15 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 440-M3
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 440-M3/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CCL19/MIP-3 beta
CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta chemokine that binds specifically to the chemokine receptor CCR‑7/EBI‑1/BLR‑2. Mouse (human) MIP-3 beta cDNA encodes a 108 (98) amino acid residue precursor protein with a predicted 25 (21) aa residue signal peptide that is cleaved to form the 83 (77) aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20 - 30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of MIP-3 beta is down‑regulated by the anti‑inflammatory cytokine IL‑10. Recombinant MIP-3 beta has been shown to be chemotactic for T cells and B cells. The MIP-3 beta receptor (CCR-7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR-7 is also strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.
References
- Kim, C.H. et al. (1998) J. Immunol. 160:2418.
- Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
- Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
- Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
Alternate Names
ELC, MIP-3 beta, MIP3 beta
Gene Symbol
CCL19
UniProt
Additional CCL19/MIP-3 beta Products
Product Documents for Recombinant Mouse CCL19/MIP-3 beta Protein
Product Specific Notices for Recombinant Mouse CCL19/MIP-3 beta Protein
For research use only
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