Recombinant Mouse CD200R4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9275-CD

R&D Systems, part of Bio-Techne
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9275-CD-050
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Key Product Details

Source

NS0

Accession #

Q6XJV4

Conjugate

Unconjugated

Applications

Bioactivity

View all CD200R4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD200 R4 protein
Thr26-Thr241, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr26

Predicted Molecular Mass

24 kDa

SDS-PAGE

49-74 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CD200 R4 is present at 0.5 μg/mL, Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) binds with a typical ED50 of 0.8-4 μg/mL.

Formulation, Preparation and Storage

9275-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD200R4

CD200 R4, also known as CD200RLa, is a transmembrane protein in the immunoglobulin superfamily (1). Mature mouse CD200 R4 consists of a 216 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and an 8 aa cytoplasmic domain (2, 3). The ECD contains one Ig-like V-type domain and one Ig-like
C2-type domain. A positively charged lysine in the transmembrane likely mediates association with the signaling molecule DAP12 (2). CD200 R4 is expressed on monocytes, bone marrow derived macrophages and dendritic cells, NK cells, and resting mast cells. It is down-regulated following Fc epsilon RI-mediated mast cell activation (2). According to conflicting reports, CD200 R4 may or may not interact with CD200 (3, 4). R&D Systems in-house testing indicates that this recombinant mouse CD200 R4 binds to recombinant mouse CD200-Fc.

References

  1. Holmannova, D. et al. (2012) Acta Medica (Hradec Kralove) 55:12.
  2. Wright, G.J. et al. (2003) J. Immunol. 171:3034.
  3. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  4. Hatherley, D. et al. (2005) J. Immunol. 175:2469.

Long Name

CD200 Receptor 4

Alternate Names

CD200 R4, F630107N04Rik, MCD200RLa

Entrez Gene IDs

239849 (Mouse)

Gene Symbol

CD200R4

UniProt

Q6XJV4

Additional CD200R4 Products

Product Documents for Recombinant Mouse CD200R4 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CD200R4 Protein, CF

For research use only

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