Recombinant Mouse CD300d Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10310-LM

R&D Systems, part of Bio-Techne
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10310-LM-050
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Key Product Details

Source

NS0

Accession #

BAF46249.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD300d Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD300d protein
Mouse CD300d
(Asn20-Leu172)
Accession # BAF46249.1		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

44 kDa

SDS-PAGE

48-66 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect is 1-10 μg/mL.

Scientific Data Images for Recombinant Mouse CD300d Fc Chimera Protein, CF

Recombinant Mouse CD300d Fc Chimera Protein Bioactivity

Recombinant Mouse CD300d Fc Chimera Protein Bioactivity

Recombinant Mouse CD300d Fc Chimera (Catalog # 10310-LM) inhibits anti-CD3 antibody induced IL-2 secretion by human T cells. The ED50 for this effect is 1‑10 μg/mL.
Recombinant Mouse CD300d Fc Chimera Protein SDS-PAGE

Recombinant Mouse CD300d Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse CD300d Fc Chimera (Catalog # 10310-LM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-66 kDa and 100-130 kDa, respectively.

Formulation, Preparation and Storage

10310-LM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300d

CD300d (also known as CD300LD or CMRF35A4) is a member of the CD300 family of transmembrane glycoproteins belonging to the immunoregulatory signaling (IRS) family. CD300d, like most CD300 family members, is found exclusively on myeloid cells, including monocyte and granulocytes (1). CD300 members contain a V-type immunoglobulin-like domain with an additional pair of cysteine residues in the extracellular domain (ECD), a transmembrane region, and a short cytoplasmic tail (2, 3). The mature ECD of human CD300d is 146 amino acids (aa) and shares a 48% and 45% identity with mouse and rat CD300d, respectively. CD300d recruits ITAM-bearing Adaptor FceRg. CD300d interacts with all CD300 family members with exception of CD300c, and plays a role in the regulation and/or formation of CD300 complexes on the cell surface and consequently modulate the state of activation of myeloid cells. CD300 family members modulate a broad and diverse array of immune cell processes via their paired activating and inhibitory receptor functions (2, 3). Mouse CD300d, along with CD300lf, has been identified as a receptor for permissive murine noroviral infection (MNoV) (4). Further, expression of murine CD300LF on human and other mammalian cell lines confers cross‑species permissively (5). Additional research into CD300 family member function during viral infections could help develop novel anti-viral therapies (6). Our in‑house data indicate that CD300D inhibits T cell activation, including anti-CD3 induced IL-2 and IFN-gamma secretion.

References

  1. Comas-Casellas, E. et al. 2011. J. Biol. Chem. 287:9682.
  2. Clark, G.J. et al. (2009) Trends in Immunology 30:209.
  3. Borrego F. (2013) Blood 121:1951.
  4. Haga, K. et al. 2016. PNAS. 113:E6248.
  5. Orchard, R. et al. 2016. Science. 353:933.
  6. Graziano, V et al. 2019. Viruses. 11:pii.

Long Name

CD300 Molecule-Like Family Member D

Alternate Names

CD300d, CD300LD, CLM-5, MAIR-IV

Entrez Gene IDs

100131439 (Human); 217305 (Mouse); 100361633 (Rat); 100686711 (Canine)

Gene Symbol

CD300LD

UniProt

BAF46249.1

Additional CD300d Products

Product Documents for Recombinant Mouse CD300d Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse CD300d Fc Chimera Protein, CF

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