Recombinant Mouse CD300e/LMIR6 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7698-LM

R&D Systems, part of Bio-Techne
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7698-LM-050
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Key Product Details

Source

CHO

Accession #

EDL34457

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD300e/LMIR6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse CD300e/LMIR6 protein
Mouse CD300E
(Leu18-Leu166)
Accession # EDL34457		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu18

Predicted Molecular Mass

43.6 kDa (monomer)

SDS-PAGE

55-60 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
The ED50 for this effect is 0.75-3.5 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

7698-LM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300e/LMIR6

CD300E, also known as LMIR6, IREM-2, and CMRF35-like 2 (CLM-2), is an approximately 35 kDa type I transmembrane glycoprotein in the LMIR family of immune regulatory proteins (1). It consists of a 154 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 4 aa cytoplasmic stub (2). Within the ECD, mouse CD300E shares 57% and 78% aa sequence identity with human and rat CD300E, respectively. CD300E is expressed on monocytes and dendritic cells (3, 4). Its transmembrane segment contains a charged lysine residue which mediates CD300E association with the signaling adaptor molecule DAP12 and enables CD300E to function as an activating receptor (2, 3). Antibody crosslinking of CD300E promotes monocyte and dendritic cell survival, production of inflammatory chemokines and cytokines, upregulation of costimulatory molecules, and also the ability of dendritic cells to induce T cell proliferation (4).

References

  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Chung, D.H. et al. (2003) J. Immunol. 141:6541.
  3. Aguilar, H. et al. (2004) J. Immunol. 173:6703.
  4. Brckalo, T. et al. (2010) Eur. J. Immunol. 40:722.

Alternate Names

CD300e, CD300LE, CLM2, IREM-2, LMIR6

Entrez Gene IDs

342510 (Human); 217306 (Mouse)

Gene Symbol

CD300E

UniProt

EDL34457

Additional CD300e/LMIR6 Products

Product Documents for Recombinant Mouse CD300e/LMIR6 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CD300e/LMIR6 Fc Chimera Protein, CF

For research use only

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