Recombinant Mouse CD43 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9959-CD

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

P15702

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD43 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD43 protein
Mouse CD43
(Asp20-Gly248)
Accession # P15702		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp20

Predicted Molecular Mass

50 kDa

SDS-PAGE

99-118 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CD43 Fc Chimera is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse Siglec‑1/CD169 Fc Chimera (Catalog # 5610-SL) that produces 50% optimal binding response is 0.1-0.8 μg/mL.

Scientific Data Images for Recombinant Mouse CD43 Fc Chimera Protein, CF

Recombinant Mouse CD43 Fc Chimera Protein Binding Activity

Recombinant Mouse CD43 Fc Chimera Protein Binding Activity

When Recombinant Mouse CD43 Fc Chimera is immobilized at 1 µg/mL, 100 µL/well, Recombinant Mouse Siglec-1/CD169 Fc Chimera (Catalog # 5610-SL) binds with an ED50 of 0.1-0.8 µg/mL.
Recombinant Mouse CD43 Fc Chimera Protein SDS-PAGE

Recombinant Mouse CD43 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse CD43 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 100-120 kDa and 200-220 kDa, respectively.

Formulation, Preparation and Storage

9959-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CD43

CD43, also known as Leukosialin, Sialophorin, B-cell differentiation antigen LP-3 and Ly-48, is a type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells (1). Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific (2). CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion (3). In mouse, CD43 is synthesized as a 395 amino acid (aa) precursor that contains a 19 aa signal sequence, a 229 aa extracellular region, a 23 aa TM domain, and a 124 aa cytoplasmic tail. The mouse extracellular region (aa 20-248) has only one N-linked glycosylation site while the human extracellular region (aa 20-253) has 25 O-linked glycosylation sites and one N-linked glycosylation site. Mouse CD43 extracellular region shares 38% and 61% aa sequence identity with the extracellular regions in human and rat CD43, respectively.

References

  1. Santamaría, M. et al. (1996). Cancer Res. 56:3526.
  2. Manjunath, N. et al. (1995) Nature 377:535.
  3. Brown, T. J. et al. (1996) J. Biol. Chem. 271:27686.

Long Name

Cluster of Differentiation 43

Alternate Names

CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN

Entrez Gene IDs

6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)

Gene Symbol

SPN

UniProt

P15702

Additional CD43 Products

Product Documents for Recombinant Mouse CD43 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CD43 Fc Chimera Protein, CF

For research use only

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