Recombinant Mouse CD6 His Tagged Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9946-CD

R&D Systems, part of Bio-Techne
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9946-CD-100
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Key Product Details

Source

NS0

Accession #

Q61003-1

Conjugate

Unconjugated

Applications

Bioactivity

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Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD6 protein
Leu18-Gly396, with a C-Terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu18

Predicted Molecular Mass

42 kDa

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells.
The ED50 for this effect is 0.4-2.4 μg/mL.

Scientific Data Images for Recombinant Mouse CD6 His Tagged Protein, CF

Recombinant Mouse CD6 His Tagged Protein Bioactivity

Recombinant Mouse CD6 His Tagged Protein Bioactivity

Immobilized Recombinant Mouse CD6 (Catalog # 9946-CD) supports the adhesion of HuT 78 human cutaneous T cell lymphoma cells. The ED50 for this effect is 0.4-2.4 μg/mL.
Recombinant Mouse CD6 His Tagged Protein SDS-PAGE

Recombinant Mouse CD6 His Tagged Protein SDS-PAGE

2 μg/lane of Recombinant Mouse CD6 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 70 - 80 kDa.

Formulation, Preparation and Storage

9946-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CD6

CD6 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, which is characterized by the presence of one or several repeats of SRCR domains in their extracellular region (1). CD6 is a type I transmembrane glycoprotein and contains three extracellular SRCR domains (2, 3). It is expressed on thymocytes, T cells, a subset of B cells, and on certain regions of the brain (3, 4). Mouse CD6 is a 665 amino acid (aa) protein that includes a 16 aa signal sequence, a 382 aa extracellular domain, a 21 aa transmembrane segment, and a 246 aa cytoplasmic region (5, 6). Within the ECD, mouse CD6 shares 69% and 88% aa sequence identity with human and rat CD6, respectively. CD6 appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. Studies demonstrating a mitogenic effect for T cells with some CD6-specific monoclonal antibodies, in conjunction with either accessory cells or PMA and anti-CD2 mAb, support the concept of CD6 as a co-stimulatory molecule (7-12). Additionally, anti-CD6 monoclonal antibody has been used as an immunosuppressive agent for patients undergoing kidney or bone marrow allograft rejection. It has also been used to remove CD6+ T cells from donor bone marrow prior to allogenic bone marrow transplantation. Other studies have demonstrated an adhesive role for CD6. It has been demonstrated to bind the activated leukocyte cell adhesion molecule (ALCAM, CD166). CD6/ALCAM interactions have been postulated to play a role in thymocyte development (9, 13). Additionally, the presence of ALCAM on neuronal cells may provide a mechanism of interaction between CD6+ T cell and ALCAM+ neuronal cells. Phosphorylation of the CD6 molecule appears to play a role in CD6-mediated signal transduction (9, 13). Serine and threonine residues become hyperphosphorylated and tyrosine residues become phosphorylated when T cells are activated with anti-CD6 mAb in conjunction with PMA, anti-CD2, or anti-CD3 mAb (8, 10, 11, 14). The CD6 intracellular domain contains regions that can interact with SH2 or SH3-containing proteins. However, the signaling pathways have not been elucidated (5, 15, 16).

References

  1. Sarrias, M. et al. (2007) Proc. Natl. Acad. Sci. USA. 104:11724.
  2. Chappell, P. et al. (2015) Structure. 23:1426.
  3. Whitney, G.S. et al. (1995) J. Biol. Chem. 270:18187.
  4. Mayer, B. et al. (1990) J. Neuroimmunol. 29:193.
  5. Robinson, W.H. et al. (1995) J. Immunol. 155:4739.
  6. Aruffo, A. et al. (1997) Immunol. Today 18:498.
  7. Gangemi, R. et al. (1989) J. Immunol. 143:2439.
  8. Swack, J.A. et al. (1991) J. Biol. Chem. 266:7137.
  9. Starling, G.C. et al. (1996) Eur. J. Immunol. 26:738.
  10. Swack, J.A. et al. (1989) Mol. Immunol. 26:1037.
  11. Pawelec, G. and H.J. Buhring (1991) Human Immunol. 31:165.
  12. Singer, N.G. et al. (1996) Immunology 88:537.
  13. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  14. Osorio, L.M. et al. (1995) Cell Immunol. 166:44.
  15. Robinson, W.H. et al. (1995) Eur. J. Immunol. 25:2765.
  16. Whitney, G. et al. (1995) Mol. Immunol.  32:89.

Alternate Names

CD6

Entrez Gene IDs

923 (Human); 12511 (Mouse); 102143944 (Cynomolgus Monkey)

Gene Symbol

CD6

UniProt

Q61003-1

Additional CD6 Products

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