Recombinant Mouse CD7 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8145-CD

R&D Systems, part of Bio-Techne
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8145-CD-050
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Key Product Details

Source

NS0

Accession #

P50283

Conjugate

Unconjugated

Applications

Bioactivity

View all CD7 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD7 protein
Gln24-Pro150, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln24 predicted

Predicted Molecular Mass

15 kDa

SDS-PAGE

20-35 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse SECTM1 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse CD7 that produces 50% optimal binding response is approximately 15‑75 ng/mL.

Formulation, Preparation and Storage

8145-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD7

CD7, also known as Leu-9, is an approximately 40 kDa glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily (1). Mature mouse CD7 consists of a 127 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 39 aa cytoplasmic domain (2). Within the ECD, mouse CD7 shares 43% and 81% aa sequence identity with human and rat CD7, respectively. CD7 is expressed on T cells (2, 3), NK cells (4), myeloid progenitor cells (5), and CD19+ B progenitor cells (6). Among CD8+ T cells, the CD7-bright population preferentially contains naïve and memory cells, while more weak expressors are primarily effector cells (7). CD7 associates in cis with CD3 and CD45 and in trans with SECTM1 (8, 9). Ligation of CD7 with antibodies or SECTM1 co-stimulates the activation and proliferation of CD4+ and CD8+ T cells (10, 11) and NK cells (4, 9). It also enhances integrin-mediated adhesion of these cells to Fibronectin, ICAM-1, and VCAM-1 (3, 12). CD7 additionally mediates the Galectin-1 induced apoptosis of T cells (13) and facilitates the fusion of HIV-1 with target cells (14).

References

  1. Sutherland, D.R. et al. (1984) J. Immunol. 133:327.
  2. Yoshikawa, K. et al. (1993) Immunogenetics 37:114.
  3. Shimizu, Y. et al. (1992) J. Exp. Med. 175:577.
  4. Rabinowich, H. et al. (1994) J. Immunol. 152:517.
  5. Chabannon, C. et al. (1992) J. Immunol. 149:2110.
  6. Grumayer, E.R. et al. (1991) Blood 77:64.
  7. Aandahl, E.M. et al. (2003) J. Immunol. 170:2349.
  8. Lazarovits, A.I. et al. (1994) J. Immunol. 153:3956.
  9. Lyman, S.D. et al. (2000) J. Biol. Chem. 275:3431.
  10. Wang, T. et al. (2012) J. Leukoc. Biol. 91:449.
  11. Carrera, A.C. et al. (1988) J. Immunol. 141:1919.
  12. Rabinowich, H. et al. (1994) J. Immunol. 153:3504.
  13. Pace, K.E. et al. (2000) J. Immunol. 165:2331.
  14. Sato, A.I. et al. (1994) J. Immunol. 152:5142.

Alternate Names

CD7, LEU-9, TP41

Entrez Gene IDs

924 (Human); 12516 (Mouse); 303747 (Rat); 102124399 (Cynomolgus Monkey)

Gene Symbol

CD7

UniProt

P50283

Additional CD7 Products

Product Documents for Recombinant Mouse CD7 Protein, CF

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Product Specific Notices for Recombinant Mouse CD7 Protein, CF

For research use only

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