Recombinant Mouse CD84/SLAMF5 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7459-CD

R&D Systems, part of Bio-Techne
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7459-CD-050
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Key Product Details

Source

NS0

Accession #

Q18PI6

Conjugate

Unconjugated

Applications

Bioactivity

View all CD84/SLAMF5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD84/SLAMF5 protein
Ala20-Pro223, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala20

Predicted Molecular Mass

23.7 kDa

SDS-PAGE

35-41 kDa, reducing conditions

Activity

Meassure by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3+ T cells.
The ED50 for this effect is 0.4-2 μg/mL.

Formulation, Preparation and Storage

7459-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD84/SLAMF5

CD84, also known as Ly‑9B and SLAMF5, is a type I transmembrane protein in the SLAM subgroup of the CD2 family. SLAM family proteins regulate multiple aspects of immune system function (1). Mature mouse CD84 consists of a 200 amino acid (aa) extracellular domain (ECD) with two Ig‑like domains, a 21 aa transmembrane segment, and a 87 aa cytoplasmic domain with two immunoreceptor tyrosine‑based switch motifs (ITSMs) (2). Alternate splicing generates an additional isoform that is truncated following the first Ig‑like domain. CD84 exhibits homophilic binding which is mediated by the N‑terminal Ig‑like domain (3). Ligation induces tyrosine phosphorylation in the cytoplasmic ITSMs which then recruit the signaling adaptor molecules SAP (SLAM‑associated protein) and EAT‑2 (EWS/Fli1‑activated transcript 2) (4, 5). CD84 is expressed as a 60‑90 kDa molecule with extensive and cell type‑specific glycosylation (6‑8). It is widely expressed among hematopoietic cells including hematopoietic stem cells (8), myeloid cells (e.g. macrophages, monocytes, dendritic cells, granulocytes, and mast cells) (4, 7‑10), platelets and megakaryocytes (3, 7, 8, 11), and lymphocytes. Within the T cell lineage, CD84 is expressed on thymocytes, CD4-CD8- cells, single positive CD4 or CD8 cells, NKT cells, and on mouse but not human NK cells (3, 4, 8, 9, 12). Within the B cell lineage, it is expressed on pro‑ and pre‑, mature, marginal zone, and memory B cells as well as plasma cells (4, 8, 13). CD84 signaling inhibits Fc epsilon RI-induced mast cell activation (10) but enhances platelet activation (11), LPS‑induced macrophage activation (8), T cell proliferation and IFN‑ gamma production (3, 5), and the interactions between T cells and B cells that are required for germinal center formation (14).

References

  1. Cannons, J.L. et al. (2011) Annu. Rev. Immunol.  29:665.
  2. de la Fuente, M.A. et al. (1999) Immunogenetics 49:249.
  3. Martin, M. et al. (2000) J. Immunol. 167:3668.
  4. Tangye, S.G. et al. (2002) Eur. J. Immunol. 32:1640.
  5. Tangye, S.G. et al. (2003) J. Immunol. 171:2485.
  6. de la Fuente, M.A. et al. (1997) Blood. 90:2398.
  7. Krause, S.W. et al. (2000) Biochem. J. 346:729.
  8. Sintes, J. et al. (2010) J. Leukoc. Biol. 88:687.
  9. Romero, X. et al. (2004) Tissue Antigens 64:132.
  10. Alvarez-Errico, D. et al. (2011) J. Immunol. 187:5577.
  11. Nanda, N. et al. (2005) Blood 106:3028.
  12. Wang, N. et al. (2010) J. Immunol. 185:5683.
  13. De Salort, J. et al. (2011) Immunol. Lett. 134:129.
  14. Cannons, J.L. et al. (2010) Immunity 32:253.

Long Name

SLAM Family Member 5

Alternate Names

CD84, Ly-9B, SLAMF5

Entrez Gene IDs

8832 (Human); 12523 (Mouse)

Gene Symbol

CD84

UniProt

Q18PI6

Additional CD84/SLAMF5 Products

Product Documents for Recombinant Mouse CD84/SLAMF5 Protein, CF

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Product Specific Notices for Recombinant Mouse CD84/SLAMF5 Protein, CF

For research use only

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