Recombinant Mouse CDCP1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9688-CU

R&D Systems, part of Bio-Techne
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9688-CU-050
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Key Product Details

Source

NS0

Accession #

Q5U462

Conjugate

Unconjugated

View all CDCP1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CDCP1 protein
Arg25-Ala667, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg25 & Ser30

Predicted Molecular Mass

73 kDa

SDS-PAGE

90-108 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9688-CU
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDCP1

CDCP1 (CUB-domain containing protein 1; also known as CD318 and SIMA135) is a novel, 135 kDa cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells (1). It is reported that this protein is over-expressed in colon and lung cancers. CDCP1 is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa 30‑667) and a phosphotyrosine site at Tyr731. The phosphorylation state of CDCP-1 has an effect on anchorage in epithelial cells (2). When unligated, CDCP1 can be proteolytically cleaved between aa 270‑300. This generates an 80 kDa TM protein that may be missing the N-terminal CUB domain (aa 221‑350) (3). Over aa 25‑667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.

References

  1. Hooper, J.D. et al. (2003) Oncogene 22:1783.
  2. Spassov, D.S. et al. (2013) Cancer Res. 73:1168.
  3. He, Y. et al. (2010) J. Biol. Chem. 285:26162.

Long Name

CUB Domain Containing Protein 1

Alternate Names

CD318, SIMA135

Entrez Gene IDs

64866 (Human); 109332 (Mouse)

Gene Symbol

CDCP1

UniProt

Q5U462

Additional CDCP1 Products

Product Documents for Recombinant Mouse CDCP1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse CDCP1 Protein, CF

For research use only

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