Recombinant Mouse Chordin-Like 2 Protein

R&D Systems, part of Bio-Techne | Catalog # 2520-CH

R&D Systems, part of Bio-Techne
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Carrier Free
2520-CH-050/CF
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With Carrier
2520-CH-050
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Key Product Details

Source

NS0

Accession #

Q8VEA6.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Chordin-like 2/CHRDL2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Chordin-like 2/CHRDL2 protein
Gln24-Leu426, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln24 predicted

Predicted Molecular Mass

46 kDa

SDS-PAGE

50-60 kDa, reducing conditions

Activity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.2-1 µg/mL in the presence of 30 ng/mL of rhBMP-4.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 2520-CH
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 2520-CH/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chordin-like 2/CHRDL2

Chordin-like 2 (CHL2, CHRDL2, or BNF-1), is an approximately 55 kDa secreted glycoprotein that acts as a BMP antagonist. Mature mouse CHL2 contains three von Willebrand type C repeats and shares 73% and 95% amino acid sequence identity with human and rat CHL2, respectively (1-3). Alternative splicing of mouse Chordin-like 2 generates additional isoforms with substituted N-terminal or C-terminal regions (3). CHL2 is expressed in chondrocytes in developing joint cartilage (2, 4), osteoblasts, myotubes, brain, some carcinomas, and the genitourinary system (e.g. uterus, prostate, testis, bladder) (1-3). CHL2 binds to multiple TGF-beta superfamily proteins including BMP-2, -4, -5, -6, -7, GDF-5, Activin A, and TSG (2, 3, 5, 6). It prevents the binding of BMPs to type 1 and type 2 receptors as well as BMP-induced cellular responses (2, 5). CHL2 reduces the rate of matrix deposition by mesenchymal cells, acting as a negative regulator of cartilage formation (2).

References

  1. Wu, I. and M.A. Moses (2003) Gene 311:105.
  2. Nakayama, N. et al. (2004) Development 131:229.
  3. Oren, A. et al. (2004) Gene 331:17.
  4. Lorda-Diez, C.I. et al. (2013) PLoS One 8:e60423.
  5. Fujisawa, T. et al. (2009) Biochem. Biophys. Res. Commun. 385:215.
  6. Zhang, J.L. et al. (2007) J. Biol. Chem. 282:20002.

Alternate Names

BNF-1, Chordin like 2, CHRDL2

Entrez Gene IDs

25884 (Human); 69121 (Mouse)

Gene Symbol

CHRDL2

UniProt

Q8VEA6.1

Additional Chordin-like 2/CHRDL2 Products

Product Documents for Recombinant Mouse Chordin-Like 2 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 2520-CH (with carrier)
Download SDS 2520-CH/CF (carrier free)

Product Specific Notices for Recombinant Mouse Chordin-Like 2 Protein

For research use only

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