Recombinant Mouse CKAP4/p63 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9734-CK

R&D Systems, part of Bio-Techne
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9734-CK-050
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Key Product Details

Source

NS0

Accession #

AAH25522

Conjugate

Unconjugated

Applications

Bioactivity

View all CKAP4/p63 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CKAP4/p63 protein
Val109-Ile575, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

53 kDa

SDS-PAGE

45-59 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Dkk‑1 (Catalog # 5897-DK) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse CKAP4/p63 that produces 50% of the optimal binding response is 35-280 ng/mL.

Scientific Data Images for Recombinant Mouse CKAP4/p63 Protein, CF

Recombinant Mouse CKAP4/p63 Protein Binding Activity

Recombinant Mouse CKAP4/p63 Protein Binding Activity

When Recombinant Mouse Dkk-1 (Catalog # 5897-DK) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse CKAP4/p63 binds with an ED50 of 35-280 ng/mL.

Formulation, Preparation and Storage

9734-CK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CKAP4/p63

CKAP4 (Cytoskeleton-associated protein 4; also CLIMP63 and p63) is a 63-64 kDa molecule that belongs to no known protein family. It is found intracellularly, and on the plasma membrane of select cells such as vascular smooth muscle and Type II Greater alveolar lung cells (1). CKAP4 has a bimodal distribution. First, it is embedded in the membrane of a compartment that links the ER with the Golgi apparatus (2). This localization is dependent upon its ability to form homooligomers, and its presence serves to anchor microtubules and direct the formation of tubular ER. Second, it is embedded in the plasma membrane and serves as a receptor for SP-A/surfactant protein-A (in lung) (2) and tPA (in vessels) (3). Mouse CKAP4 is a 575 amino acid (aa) type II transmembrane nonglycosylated protein. It contains an 85 aa N-terminal cytoplasmic region plus a 467 aa C-terminal luminal domain (aa 109-575) (4). The luminal domain contains three coiled-coil regions (aa 125-193; 236-438; 507-575) plus three utilized phosphorylation sites (5). CKAP4 undergoes reversible palmitoylation (6). Over aa 109-575, mouse CKAP4 shares 82% and 94% aa sequence identity with human and rat CKAP4, respectively. CKAP4 has been shown to be required for APF-mediated signaling (6-9), for gentamycin-induced cytotoxicity (8), for regulation of tPA function (3), and for SP-A-induced surfactant uptake by pneumocytes (10). CKAP4 interacts with Dickkopf-1 (Dkk-1) to promote activation of AKT-dependent, Wnt-independent signaling(11). Both CKAP4 and Dkk-1 are frequently up-regulated in pancreatic and lung cancers (11). Dkk-1 binds to CKAP4 with high affinity that is comparable to but independent of its interaction with LRP5/6 (11).

References

  1. Bates, S. R. et al. (2008) Am J Physiol Lung Cell Mol Physiol, 295:L658.
  2. Gupta, N. et al. (2006). Am J Physiol Lung Cell Mol Physiol 291:L436.
  3. Razzaq, T. M. et al. (2003). J. Biol. Chem. 278:42679.
  4. Schweizer, A. et al. (1994). J. Cell Biol.126:25.
  5. Huttlin, E. L. et al. (2010) Cell 143:1174.
  6. Planey, S. L. et al. (2009). Mol Biol Cell. 20:1454.
  7. Conrads, T.P. (2006) J Bio Chem, 281:49.
  8. Matika, C.A. (2012) Mol Bio Cell, 23:10.
  9. Shahjee, H.M. (2010) J Exp and Clin Cancer Res, 29:1.
  10. Kazi, A.S. (2010) Am J Physiol Lung Cell Mol Physiol 299:6.
  11. Kimura, H. et al. (2016) J Clin Invest, 126:2689.

Long Name

Cytoskeleton-associated Protein-4/Climp-63

Alternate Names

CLIMP-63, ERGIC-63, p63

Entrez Gene IDs

10970 (Human); 216197 (Mouse); 362859 (Rat)

Gene Symbol

CKAP4

UniProt

AAH25522

Additional CKAP4/p63 Products

Product Documents for Recombinant Mouse CKAP4/p63 Protein, CF

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Product Specific Notices for Recombinant Mouse CKAP4/p63 Protein, CF

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