Recombinant Mouse CLEC9a Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6776-CL

R&D Systems, part of Bio-Techne
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6776-CL-050
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Key Product Details

Source

CHO

Accession #

NP_001192292

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CLEC9a Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse CLEC9a protein
MDP Mouse IgG2A
(Glu98-Lys330)
 IEGR Mouse CLEC9a
(Lys57-Ile264)
Accession # NP_001192292
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

50.8 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by flow cytometry for its ability to bind a ligand expressed in necrotic EL-4 mouse lymphoblast cells.
When 100 ng of Recombinant Mouse CLEC9a Fc Chimera is added to 5 x 105 EL-4 cells, >50% of necrotic EL-4 cells bind to the protein.

Formulation, Preparation and Storage

6776-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC9a

CLEC9A (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C‑type lectin superfamily (1). Mature mouse CLEC9A consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 182 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD) (2‑4). Within the ECD, mouse CLEC9A shares 57% and 80% aa sequence identity with human and rat CLEC9A, respectively. Alternative splicing of mouse CLEC9A generates additional isoforms with insertions in the stalk region or deletions of the transmembrane segment, the CTLD, or a portion of the CTLD (2). Although the CTLD of CLEC9A structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9A is expressed as a disulfide‑linked homodimer of approximately 50 kDa N‑glycosylated subunits (2, 4). Human CLEC9A expression is restricted to a subpopulation of BDCA‑3+ conventional dendritic cells (cDC) and CD16- monocytes (2‑5). BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules (6). In mouse, CLEC9A is additionally expressed on plasmacytoid dendritic cells (3, 4). CLEC9A ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming (2‑5, 7). Its recognition of filamentous actin in dead cells is important for triggering an immune response to necrotic cell debris (8-10). CLEC9A is also required for the presentation of viral proteins and the subsequent CTL‑mediated killing of virus-infected cells (11, 12). CLEC9A signaling triggers activation of the tyrosine kinase Syk (2, 8).

References

  1. Kerrigan, A.M. and G.D. Brown (2010) Immunol. Rev. 234:335.
  2. Huysamen, C. et al. (2008) J. Biol. Chem. 283:16693.
  3. Caminschi, I. et al. (2008) Blood 112:3264.
  4. Sancho, D. et al. (2008) J. Clin. Invest. 118:2098.
  5. Schreibelt, G. et al. (2012) Blood 119:2284.
  6. Dudziak, D. et al. (2007) Science 315:107.
  7. Poulin, L.F. et al. (2010) J. Exp. Med. 207:1261.
  8. Sancho, D. et al. (2009) Nature 458:899.
  9. Ahrens, S. et al. (2012) Immunity 36:635.
  10. Zhang, J.-G. et al. (2012) Immunity 36:646.
  11. Iborra, S. et al. (2012) J. Clin. Invest. 122:1628.
  12. Zelenay, S. et al. (2012) J. Clin. Invest. 122:1615.

Long Name

C-type Lectin-like Receptor 9

Alternate Names

CD370, DNGR1

Entrez Gene IDs

283420 (Human); 232414 (Mouse)

Gene Symbol

CLEC9A

UniProt

NP_001192292

Additional CLEC9a Products

Product Documents for Recombinant Mouse CLEC9a Fc Chimera Protein, CF

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