Recombinant Mouse Common gamma Chain Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 784-MR
Key Product Details
Source
Sf 21 (baculovirus)
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Common gamma Chain/IL-2 R gamma protein
| Mouse Common gamma Chain (Ser25-Ala263) Accession # Q3UPA9 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser25
Predicted Molecular Mass
54.5 kDa (monomer)
SDS-PAGE
60 kDa, reducing conditions
Activity
Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells.
The ED50 for this effect is 2-10 µg/mL in the presence of 30 µg/mL of soluble IL-2 R beta and 10 ng/mL of recombinant human IL-2.
The ED50 for this effect is 2-10 µg/mL in the presence of 30 µg/mL of soluble IL-2 R beta and 10 ng/mL of recombinant human IL-2.
Formulation, Preparation and Storage
784-MR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Common gamma Chain/IL-2 R gamma
The gamma chain of the high affinity functional mouse IL-2 receptor complex belongs to the hematopoietin receptor family. Mouse Common gamma Chain is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 241 residue extracellular domain, a 21 residue transmembrane domain and an 85 residue cytoplasmic domain. Although Common gamma Chain by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma is also designated the common gamma chain ( gammac) (1, 2). The site of molecular defects in human X-linked SCID (severe combined immunodeficiency) has been mapped to the human common gamma gene.
References
- Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
- Noguchi, M. et al. (1993) Science 262:1877.
Long Name
IL-2 Receptor gamma
Alternate Names
CD132, IL-2 R gamma, IL2R gamma, IL2RG
Gene Symbol
IL2RG
UniProt
Additional Common gamma Chain/IL-2 R gamma Products
Product Documents for Recombinant Mouse Common gamma Chain Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse Common gamma Chain Fc Chimera Protein, CF
For research use only
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