Recombinant Mouse COMP Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8958-CP

R&D Systems, part of Bio-Techne
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8958-CP-050
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Key Product Details

Source

NS0

Accession #

Q9R0G6

Structure / Form

Disulfide-linked pentamer

Conjugate

Unconjugated

Applications

Bioactivity

View all COMP/Thrombospondin-5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse COMP/Thrombospondin-5 protein
Gln20-Val755, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln20 inferred from enzymatic pyroglutamate treatment revealing Gly21

Predicted Molecular Mass

81 kDa

SDS-PAGE

85-115 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.04-0.6 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 8958-CP in the following applications:

Scientific Data Images for Recombinant Mouse COMP Protein, CF

Recombinant Mouse COMP Protein Binding Activity

Recombinant Mouse COMP Protein Binding Activity

Recombinant Mouse COMP/Thrombospondin-5 (Catalog # 8958-CP) induces adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.15-0.9 μg/mL.

Formulation, Preparation and Storage

8958-CP
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COMP/Thrombospondin-5

Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). Mouse COMP contains a non-collagenous coiled-coil domain, four EGF-like repeats, eight TSP type-3 repeats, and a globular TSP C-terminal domain (5). It shares 92% and 98% aa sequence identity with human and rat COMP, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin  alpha5 beta1, although when reduced and in the absence of calcium, attachment is mediated via Integrin  alphaV beta3 (9). COMP is up-regulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type-3 repeat, results in diminished calcium binding ability (13, 14).

References

  1. Adams, J.C. and J. Lawler (2004) Int J. Biochem. Cell Biol. 36:961.
  2. Posey, K.L. et al. (2014) Matrix Biol. 37:167.
  3. Adams, J.C. (2004) Int. J. Biochem. Cell Biol. 36:1102.
  4. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
  5. Fang, C. et al. (2000) J. Orthop. Res. 18:593.
  6. DiCesare, P. et al. (1995) J. Orthopaedic Res. 13:422.
  7. Efimov, V.P. et al. (1994) FEBS Lett. 341:54.
  8. Ozbek, S., et al. (2002) EMBO J. 21:5960.
  9. Chen, F.H., et al. (2005) J. Biol. Chem. 280:32655.
  10. Wislowska, M. and B. Jablonska (2005) Clin. Rheumatol. 24:278.
  11. Xiao, Y. et al. (2004) J. Gastroenterol. Hepatol. 19:296.
  12. Liao, Q. et al. (2003) Scand. J. Gastroenterol. 38:207.
  13. Kennedy, J., et al. (2005) Eur. J. Hum. Genet. 13:547.
  14. Hou, J. et al. (2000) Cell Calcium 27:309.

Long Name

Cartilage Oligomeric Matrix Protein

Alternate Names

EDM1, EPD1, MED, PSACH, Thrombospondin-5, Thrombospondin5

Entrez Gene IDs

1311 (Human); 12845 (Mouse)

Gene Symbol

COMP

UniProt

Q9R0G6

Additional COMP/Thrombospondin-5 Products

Product Documents for Recombinant Mouse COMP Protein, CF

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Product Specific Notices for Recombinant Mouse COMP Protein, CF

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