Recombinant Mouse Complement Component C3a Protein, CF Best Seller

R&D Systems, part of Bio-Techne | Catalog # 8085-C3

R&D Systems, part of Bio-Techne
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8085-C3-025
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Key Product Details

Source

E. coli

Accession #

P01027

Conjugate

Unconjugated

Applications

Bioactivity

View all Complement Component C3a Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse Complement Component C3a protein
Ser671-Arg748

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser671

Predicted Molecular Mass

9.2 kDa

SDS-PAGE

9 kDa, reducing conditions

Activity

Measured by its ability to enhance IL-6 secretion by human peripheral blood mononuclear cells (PBMC). Fischer, W.H. et al. (1999) J. Immunol. 162:453.
The ED50 for this effect is typically 0.8-4 µg/mL

Reviewed Applications

Read 1 review rated 4 using 8085-C3 in the following applications:

Formulation, Preparation and Storage

8085-C3
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C3a

C3a is an anaphylotoxin polypeptide comprising amino acids (aa) 671-748 of the Complement C3 precursor protein (1-4). Anaphylatoxins are proteolytically generated from the C3, C4 and C5 alpha chains by convertases formed by other complement fragments (2). They share 30-36% aa identity, and mediate inflammatory responses that vary in strength in the order C5a > C3a > C4a (2). Like C4a and C5a, the 78 aa, 9 kDa mouse C3a contains six conserved cysteine residues that form a knot structure and possess an overall basic charge (4, 5). It is not glycosylated (4). The C-terminal regions of C3a and C4a show antimicrobial activity, while C5a is chemotactic but not antimicrobial (5). Mouse C3a shares 94% aa sequence identity with rat, and 65-69% with human, guinea pig, bovine, porcine and canine C3a. C3a formation is common to all three pathways of complement activation: classical (antibody-mediated), lectin and alternative (1, 2). It binds the G-protein coupled C3a receptor (C3aR) on myeloid peripheral blood leukocytes, and on activated lymphocytes, endothelial and internal organ epithelial cells (6, 7). C3a contributes to both innate and adaptive immunity. It activates mast cells and neutrophils, triggering robust mast cell degranulation in airways during asthmatic allergen challenges (8). It enhances lipopolysaccharide-induced prostaglandin, cytokine and chemokine secretion by macrophages and other cells (1, 6, 9, 10). It assists in Th2-type inflammatory reactions, promotes Th1 cell maturation, and down-regulates regulatory T cell differentiation (8, 11). It stimulates leukocyte chemotaxis and smooth muscle contraction (8, 9).  Endogenous carboxypeptidase-N can remove the arginine at the C-terminus of the anaphylatoxins to create desArg forms (1). C3adesArg, also called ASP (Acylation-Stimulating Protein) is an adipocyte-derived protein that binds the C5L2 receptor (GPR77) and stimulates adipose tissue triglyceride synthesis (2, 7, 12). The anaphylactic activity of ASP is weaker than that of C3a (7, 10). C5L2 is also involved in C3a and C5a activity (12).

References

  1. Markiewski, M. and J.D. Lambris (2007) Am. J. Pathol. 171:715.
  2. Haas, P-J. and J. van Strijp (2007) Immunol. Res. 37:161.
  3. Domdey, H. et al. (1982) Proc. Natl. Acad. Sci. USA 79:7619.
  4. Hugli, T.E. (1975) J. Biol. Chem. 250:8293.
  5. Pasupuleti, M. et al. (2007) J. Biol. Chem. 282:2520.
  6. Thurman, J.M. et al. (2007) J. Immunol. 178:1819.
  7. Kalant, D. et al. (2005) J. Biol. Chem. 280:23936.
  8. Ali, H. and R.A. Panettieri (2005) Respir. Res. 6:19.
  9. Honczarenko, M. et al. (2005) J. Immunol. 175:3698.
  10. Fischer, W.H. et al. (1999) J. Immunol. 162:453.
  11. van der Touw, W. et al. (2013) J. Immunol. 190:5921.
  12. Chen, N-J. et al. (2007) Nature 446:203.

Alternate Names

Anaphylatoxin, C3

Entrez Gene IDs

718 (Human); 12266 (Mouse)

Gene Symbol

C3

UniProt

P01027

Additional Complement Component C3a Products

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Product Specific Notices for Recombinant Mouse Complement Component C3a Protein, CF

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