Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 48-109)
R&D Systems, part of Bio-Techne | Catalog # 1091-CK
Key Product Details
Product Specifications
Source
E. coli-derived mouse CXCL7/Thymus Chemokine-1 protein
Ile48-Ile109
Ile48-Ile109
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ile48
Predicted Molecular Mass
6.8 kDa
Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CXCR2.
The ED50 for this effect is 1-5 ng/mL.
The ED50 for this effect is 1-5 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1091-CK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 1091-CK/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CXCL7/Thymus Chemokine-1
CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid residue precursor protein that shares 72% amino acid sequence identity with rat CXCL7 (accession number AAK30166). The recombinant mouse CXCL7 produced by R&D Systems has been shown to chemoattract human CXCR2 transfected mouse BaF/3 cells, but not human CXCR1 transfected mouse BaF3 cells. Two isoforms of mouse CXCL7 have been produced at R&D Systems. The Ile48 - Ile109 mouse CXCL7 isoform (R&D Systems, Catalog # 1091-CK) has been shown to be 200-fold more potent than the Lys40 - Tyr113 isoform (R&D Systems, Catalog # 763-CK) as a chemoattractant for mouse BaF/3 cells transfected with human CXCR2.
Alternate Names
Thymus Chemokine1
Gene Symbol
PPBP
UniProt
Additional CXCL7/Thymus Chemokine-1 Products
Product Documents for Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 48-109)
Product Specific Notices for Recombinant Mouse CXCL7/Thymus Chemokine-1 (aa 48-109)
For research use only
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