Recombinant Mouse Cystatin B Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1409-PI
Key Product Details
Source
E. coli
Accession #
Structure / Form
Monomer and a disulfide-linked multimer
Conjugate
Unconjugated
Applications
Inhibition Activity
Product Specifications
Source
E. coli-derived mouse Cystatin B protein
Met1-Phe98, with an N-terminal Met and 6-His tag
Met1-Phe98, with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
12 kDa (monomer)
SDS-PAGE
13 kDa, reducing conditions
Activity
Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (Catalog # ES009).
The IC50 value is <15 nM, as measured under the described conditions.
The IC50 value is <15 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1409-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM Tris and 100 mM NaCl, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cystatin B
Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Cystatin B-deficient mice have increased expression of proteolysis, apoptosis and glial activation genes, which is consistent with the pathology found in the mouse model of human progressive myoclonus epilepsy (EPM1) (3). The mouse Cystatin B consists of 98 amino acid residues (4).
References
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Pol, E. and I. Bjork (1999) Biochemistry 38:10519.
- Lieuallen, K. et al. (2001) Hum. Mol. Genet. 10:1867.
- Pennacchio, L.A. and R.M. Myers (1996) Genome Res. 6:1103.
Alternate Names
CSTB, Stefin B
Gene Symbol
CSTB
UniProt
Additional Cystatin B Products
Product Documents for Recombinant Mouse Cystatin B Protein, CF
Product Specific Notices for Recombinant Mouse Cystatin B Protein, CF
For research use only
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