Recombinant Mouse Cystatin E/M Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1284-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Cystatin E/M protein
Glu29-Val149 & Thr35-Val149, both with a C-terminal 10-His tag
Glu29-Val149 & Thr35-Val149, both with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu29 & Thr35
Predicted Molecular Mass
19 kDa
SDS-PAGE
20 kDa, reducing conditions
Activity
Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (Catalog # ES009).
The IC50 value is <7 nM, as measured under the described conditions.
The IC50 value is <7 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1284-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl, pH 6.5. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cystatin E/M
Cystatin E/M is a member of family 2 of the cystatin superfamily (1‑6). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice with a null mutation in the cystatin E/M gene have defects in epidermal cornification and die between 5 and 12 days of age (7). Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation (8). The mouse cystatin E/M has not been characterized and is shown here to inhibit papain. In addition to being a cysteine protease inhibitor, the human cystatin E/M is also a substrate for transglutaminases (9).
References
- Carninci, P. and Y. Hayashizaki (1999) Meth. Enzymol. 303:19.
- Carninci, P. et al. (2000) Genome Res. 10:1617.
- Shibata, K. et al. (2000) Genome Res. 10:1757.
- Kawai, J. et al. (2001) Nature 409:685.
- Sotiropoulou, G. et al. (1997) J. Biol. Chem. 272:903.
- Ni, J. et al. (1997) J. Biol. Chem. 272:10853.
- Zeeuwen, P.L. et al. (2002) Hum. Mol. Genet. 11:2867.
- Hong, J. et al. (2002) J. Neurochem. 81:922.
- Zeeuwen, P.L. et al. (2001) J. Invest. Dermatol. 116:693.
Alternate Names
CST6
Gene Symbol
CST6
UniProt
Additional Cystatin E/M Products
Product Documents for Recombinant Mouse Cystatin E/M Protein, CF
Product Specific Notices for Recombinant Mouse Cystatin E/M Protein, CF
For research use only
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