Recombinant Mouse DcTRAILR1/TNFRSF23 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8479-DT

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
8479-DT-025
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q9ER63

Conjugate

Unconjugated

Applications

Bioactivity

View all DcTRAILR1/TNFRSF23 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse DcTRAIL R1/TNFRSF23 protein
Met30-Ser156, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met30

Predicted Molecular Mass

15 kDa

SDS-PAGE

23-30 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse DcTRAIL R1/TNFSF23 is immobilized at 0.1 μg/mL (100 μL/well), the concentration of Recombinant Mouse TRAIL/TNFSF10 (Catalog # 1121-TL) that produces 50% optimal binding response is approximately 2-12 ng/mL.

Formulation, Preparation and Storage

8479-DT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DcTRAILR1/TNFRSF23

Decoy TRAIL receptor 1 (DcTRAIL R1), also called TNFRSF23 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein that binds TRAIL/TNFSF10 with high affinity (1). Mouse DcTRAIL R1 consists of a 29 amino acid (aa) signal peptide, a 126 aa mature region and a 21 aa GPI anchor signal. The mature region shows a TNFR cysteine rich domain (CRD) with three tandem A1/B2 repeats (2). Compared to signaling TRAIL receptors, DcTRAIL R1 does not trigger death signaling due to its lack of an intracellular death domain. Also, unlike many TNF receptors family members, DcTRAIL R1 does not contain a TRAFF-binding motif. Thus DcTRAIL R1 failed to induce NF kappaB activity. Structural studies revealed a strict species selectivity of the mouse DcTRAIL R1 for mouse and not human TRAIL based on amino acid specific electrostatic interactions between the two proteins (2). Mouse DcTRAIL R1, DcTRAIL R2 (TNFRSF22), and TNFRSF26 are encoded by clustered genes within the Kcnq1 domain of chromosome 7. The human counterpart of the Kcnq1 domain does not encode a functional homolog of the murine DcTRAIL R1 (3). Mouse DcTRAIL R1 is weakly expressed on CD4+CD25+ Tregs but not on CD4+CD25− conventional T cells. The difference in DcTRAIL R1 expression between the two T cell subsets may affect their responsiveness to TRAIL (4).

References

  1. Truneh, A. et al. (2000) J. Biol. Chem. 275:23319.
  2. Schneider, P. et al. (2003) J. Biol. Chem. 278:5444.
  3. de la Casa Esperon, E. et al. (2012) Epigenetics 7:626.
  4. Ikeda, T. et al. (2010) J. Immunol. 185:5259.

Long Name

Decoy TRAIL Receptor 1

Alternate Names

DcTRAIL R1, SOB, TNFRH1, TNFRSF23

Entrez Gene IDs

79201 (Mouse)

Gene Symbol

TNFRSF23

UniProt

Q9ER63

Additional DcTRAILR1/TNFRSF23 Products

Product Documents for Recombinant Mouse DcTRAILR1/TNFRSF23 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse DcTRAILR1/TNFRSF23 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.