Recombinant Mouse DDR2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7479-DR

R&D Systems, part of Bio-Techne
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7479-DR-050
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Key Product Details

Source

NS0

Accession #

NP_072075

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all DDR2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse DDR2 protein
Mouse DDR2
(Gln24-Arg399)
Accession # NP_072075		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln24 predicted: No results obtained, sequencing might be blocked

Predicted Molecular Mass

69.6 kDa (monomer)

SDS-PAGE

80-90 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind Recombinant Mouse DDR2 Fc Chimera with an apparent KD <10 nM.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

7479-DR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DDR2

DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin‑like domain containing subfamily of receptor tyrosine kinases (1). Mature mouse DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 433 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, mouse DDR2 shares 50% aa sequence identity with DDR1. It shares 97% and 99% aa sequence identity with human and rat DDR2, respectively. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3‑5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding. The D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a noncovalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces up‑regulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). It promotes osteoblast and chondrocyte differentiation and supports ovulation and spermatogenesis (11‑13). DDR2 is up‑regulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell overgrowth diseases (8, 10, 14, 15).

References

  1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
  2. Karn, T. et al. (1993) Oncogene 8:3433.
  3. Vogel, W. et al. (1997) Mol. Cell 1:13.
  4. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
  5. Leitinger, B. and Kwan, A.P.L. (2006) Matrix Biol. 25:355.
  6. Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.
  7. Mihai, C. et al. (2006) J. Mol. Biol. 361:864.
  8. Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.
  9. Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.
  10. Xu, L. et al. (2005) J. Biol. Chem. 280:548.
  11. Zhang, Y. et al. (2011) J. Bone Miner. Res. 26:604.
  12. Matsumura, H. et al. (2009) Physiol. Genomics 39:120.
  13. Kano, K. et al. (2010) Mol. Reprod. Dev. 77:29.
  14. Wang, J. et al. (2002) J. Autoimmun. 19:161.
  15. Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.

Long Name

Discoidin Domain Receptor 2

Alternate Names

TKT, Trk3, Tyro-10

Entrez Gene IDs

4921 (Human); 18214 (Mouse)

Gene Symbol

DDR2

UniProt

NP_072075

Additional DDR2 Products

Product Documents for Recombinant Mouse DDR2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse DDR2 Fc Chimera Protein, CF

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