Recombinant Mouse Dermatopontin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5749-DP

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
5749-DP-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q9QZZ6

Conjugate

Unconjugated

Applications

Bioactivity

View all Dermatopontin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Dermatopontin protein
Gln19-Val201 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln19 predicted

Predicted Molecular Mass

22.8 kDa

SDS-PAGE

23 kDa, reducing conditions

Activity

Measured by its ability to modulate collagen fibrillogenesis.
20 µg/mL of rmDermatopontin can significantly enhance the rate of collagen fibrillogenesis.

Formulation, Preparation and Storage

5749-DP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dermatopontin

Dermatopontin, also known as TRAMP (tyrosine rich acidic matrix protein) and EQ-1 (early quiescence-1), is a widely expressed noncollagenous protein component of the extracellular matrix (1, 2). Mature mouse Dermatopontin shares 92% and 97% amino acid sequence identity with human and rat Dermatopontin, respectively. It is a 22 kDa molecule that is tyrosine sulfated but not glycosylated (3, 4). Dermatopontin contains three disulfide bonded loop structures that enclose conserved hexapeptide motifs (5). It accelerates collagen fibril formation in vitro, and Dermatopontin deficient mice exhibit altered collagen matrix deposition and organization (6 - 8). Dermatopontin is downregulated in fibrotic growths such as leiomyoma and scar tissue (9, 10). It binds both TGF-beta and the proteoglycan Decorin, interactions that can increase the bioavailability of TGF-beta (11, 12). Dermatopontin promotes bone mineralization under the control of the vitamin D receptor and inhibits BMP-2 effects on osteoblast precursors (13, 14).

References

  1. Okamoto, O. and S. Fujiwara (2006) Connect. Tissue Res. 47:177.
  2. Tzen, C.Y. and Y.W. Huang (2004) Exp. Cell Res. 294:30.
  3. Forbes, E.G. et al. (1994) FEBS Lett. 351:433.
  4. Cronshaw, A.D. et al. (1993) Matrix 13:255.
  5. Neame, P.J. et al. (1989) J. Biol. Chem. 264:5474.
  6. MacBeath, J.R.E. et al. (1993) J. Biol. Chem. 268:19826.
  7. Takeda, U. et al. (2002) J. Invest. Dermatol. 119:678.
  8. Cooper, L.J. et al. (2006) Invest. Opthalmol. Vis. Sci. 47:3303.
  9. Catherino, W.H. et al. (2004) Genes Chromosomes Cancer 40:204.
  10. Kuroda, K. et al. (1999) J. Invest. Dermatol. 112:706.
  11. Okamoto, O. et al. (1996) J. Biochem. 119:106.
  12. Okamoto, O. et al. (1999) Biochem. J. 337:537.
  13. Pochampally, R.R. et al. (2007) J. Bone Miner. Res. 22:1338.
  14. Behnam, K. et al. (2006) Connect. Tissue Res. 47:271.

Alternate Names

DPT, Eq1

Entrez Gene IDs

1805 (Human); 56429 (Mouse); 289178 (Rat)

Gene Symbol

DPT

UniProt

Q9QZZ6 (Mouse)

Additional Dermatopontin Products

Product Documents for Recombinant Mouse Dermatopontin Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Dermatopontin Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.