Recombinant Mouse Desmoglein-2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7699-DM

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
7699-DM-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

O55111

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Desmoglein-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Desmoglein-2 protein
Mouse Desmoglein-2
(Leu29-Ala618)
Accession # O55111		 IEGRMDP Mouse IgG2A
(Gln98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu29 & Ala55

Predicted Molecular Mass

92.9 kDa (monomer)

SDS-PAGE

100-120 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BUD-8 human fibroblast cells.
When 3 x 104 cells are added to Recombinant Mouse Desmoglein‑2 Fc Chimera coated plates (10 μg/mL, 100 μL/well), approximately 30%‑60% will adhere after 1 hour at 37 °C.
Optimal dilutions should be determinded by each laboratory for each application.

Formulation, Preparation and Storage

7699-DM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmoglein-2

Desmoglein-2 (DSG2) is a 160 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. It is a major protein component of desmosomal contacts between epithelial cells (1, 2). Mature mouse Desmoglein‑2 consists of a 564 amino acid (aa) extracellular domain (ECD) with four cadherin domains, a 21 aa transmembrane segment, and a 483 aa cytoplasmic domain with six desmoglein repeats (3, 4). Within the ECD, mouse Desmoglein‑2 shares 81% and 93% aa sequence identity with human and rat Desmoglein‑2, respectively. A 60 kDa ECD fragment can be proteolytically shed, and this is enhanced by EGF R activation (5‑7). The cytoplasmic domain is cleaved during apoptosis, leaving the transmembrane segment and ECD as a cell‑associated fragment (8). Desmoglein‑2 is widely expressed in epithelia and is required for maintenance of the epithelial barrier (1, 2, 9). It can be either up- or down‑regulated in a variety of carcinomas during tumor progression (10). Desmoglein‑2 also functions as a cell adhesion receptor for several adenovirus serotypes (11). This binding disrupts intercellular junctions between epithelial cells and promotes epithelial‑mesenchymal transition (EMT) (11). Mutations in Desmoglein‑2 cause cardiac dysfunction typical of arrythmogenic right ventricular cardiomyopathy (ARVC) (12).

References

  1. Brooke, M.A. et al. (2012) J. Pathol. 226:158.
  2. Thomason, H.A. et al. (2010) Biochem. J. 429:419.
  3. Mahoney, M.G. et al. (2002) Exp. Dermatol. 11:115.
  4. Whittock, N.V. (2003) J. Invest. Dermatol. 120:970.
  5. Bech-Serra, J.J. et al. (2006) Mol. Cell. Biol. 26:5086.
  6. Klessner, J.L. et al. (2009) Mol. Biol. Cell 20:328.
  7. Ramani, V.C. et al. (2008) BMC Cancer 8:373.
  8. Nava, P. et al. (2007) Mol. Biol. Cell 18:4565.
  9. Schlegel, N. et al. (2010) Am. J. Physiol. Gastrointest. Liver Physiol. 298:G774.
  10. Dusek, R.L. and L.D. Attardi (2011) Nat. Rev. Cancer 11:317.
  11. Wang, H. et al. (2011) Nat. Med. 17:96.
  12. Pilichou, K. et al. (2009) J. Exp. Med. 206:1787.

Alternate Names

CDHF5, Desmoglein2, DSG2, HDGC

Entrez Gene IDs

1829 (Human); 13511 (Mouse)

Gene Symbol

DSG2

UniProt

O55111

Additional Desmoglein-2 Products

Product Documents for Recombinant Mouse Desmoglein-2 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Desmoglein-2 Fc Chimera Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.