Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9364-EP

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9364-EP-100
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q99JW5

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all EpCAM/TROP1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse EpCAM/TROP1 protein
Mouse EpCAM/TROP1
(Gln24 & Arg81-Thr266)
Accession # Q99JW5		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg81 detected. Gln24 inferred from enzymatic pyroglutamate treatment revealing Arg25

Predicted Molecular Mass

55 kDa

SDS-PAGE

58-75 kDa and 8 kDa

Activity

Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line.
When 5 x 104 cells/well are added to Recombinant Mouse EpCAM/TROP-1 and Human Fibronectin (0.5 μg/mL, Catalog # 1918-FN) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.4-2.4 μg/mL

Scientific Data Images for Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF

Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein Bioactivity

Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein Bioactivity

Immobilized Recombinant Mouse EpCAM/TROP1 (Catalog # 9364-EP) enhances L Cells mouse fibroblasts adhesion in the presence of Human Fibronectin (Catalog # 1918-FN). The ED50 for this effect is 0.4-2.4 µg/mL.

Formulation, Preparation and Storage

9364-EP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, CD326 and TROP‑1, is a 40 kDa transmembrane glycoprotein composed of a 243 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF‑like) repeats, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In mouse, EpCAM also shares 51% aa sequence homology with Trop‑2. EpCAM is detected in basolateral cell membranes of all simple epithelia and expression has been found to increase in epithelia tissues during fetal development (1, 2). EpCAM has been shown to function as a homophilic Ca2+ independent adhesion molecule, but it does not structurally resemble any of the four major families of cell adhesion molecules (3). Homophilic adhesion via EpCAM requires the interaction of both EGF‑like repeats, with the first EGF‑like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM (4). EpCAM has been identified as a surface marker for pluripotent embryonic stem cells and is strongly associated with the maintenance of the undifferentiated state of ESCs (5). EpCAM is highly expressed on the surface of epithelial cancer cells including colon, stomach, lung, pancreas, prostate and breast (6, 7).

References

  1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
  2. Keisuke, N. et al. (2009) PLoS One 4 :e8543
  3. Litvinov, S.V. et al. (1994) J. Cell Biol. 125:437.
  4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  5. González, B. et al. (2009) Stem Cells, 27: 1782.
  6. Went, P. et al. (2006) Br. J. Cancer 94:128.
  7. Spizzo, G.S. et al. (2002) Int. J. Cancer 98:883.

Long Name

Epithelial Cell Adhesion Molecule

Alternate Names

17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1

Entrez Gene IDs

4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)

Gene Symbol

EPCAM

UniProt

Q99JW5

Additional EpCAM/TROP1 Products

Product Documents for Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse EpCAM/TROP1 Fc Chimera Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.