Recombinant Mouse EPCR Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9068-ER

R&D Systems, part of Bio-Techne
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9068-ER-050
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Key Product Details

Source

NS0

Accession #

AAC42049

Conjugate

Unconjugated

Applications

Bioactivity

View all EPCR Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse EPCR protein
Leu18-Ser214, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu18

Predicted Molecular Mass

24 kDa

SDS-PAGE

36-47 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Human Activated Protein C is immobilized at 3 µg/mL (100 µL/well), the concentration of Recombinant Mouse EPCR that produces 50% of the optimal binding response is approximately 0.1-0.6 μg/mL

Formulation, Preparation and Storage

9068-ER
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EPCR

The endothelial protein C receptor (EPCR), also known as CD201, is an approximately 50 kDa transmembrane glycoprotein expressed on vascular endothelial cells and functions as a negative regulator of thrombosis (1). Mature mouse EPCR consists of a 197 amino acid extracellular domain (ECD), a 21 aa transmembrane segment, and a 7 aa cytoplasmic tail (2). Within the ECD, mouse EPCR shares 65% and 85% aa sequence identity with human and rat EPCR, respectively. EPCR inhibits thrombosis through its interactions with Protein C, activated Protein C (APC), and Coagulation Factors VII, and VIIa (3, 4). It enhances the activation of Protein C in response to complexes of Thrombin-Thrombomodulin (5). In humans, a soluble form of EPCR can be produced by alternative splicing or ADAM17/TACE mediated shedding (6-9), and this protein inhibits the anti-coagulant activity of APC (10, 11). EPCR can be degraded on the surface of endothelial cells by Neutrophil Elastase (12). Activation of EPCR also protects vascular endothelial cells from Thrombin-induced apoptosis (13). EPCR binds to CD11b/CD18 (Mac-1) on monocytes and mediates monocyte adhesion to the vascular endothelium (14). In addition, EPCR binds to the antigen receptor on gamma delta T cells (15), promotes hematopoietic stem cell retention in the bone marrow (9), and binds to surface proteins of some species of Plasmodium, contributing to pathogenicity in severe malaria (16).

References

  1. Montes, R. et al. (2012) Thromb. Haemost. 107:815.
  2. Fukudome, K. and C.T. Esmon (1995) J. Biol. Chem. 270:5571.
  3. Fukudome, K. and C.T. Esmon (1994) J. Biol. Chem. 269:26486.
  4. Ghosh, S. et al. (2007) J. Biol. Chem. 282:11849.
  5. Stearns-Kurosawa, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:10212.
  6. Saposnik, B. et al. (2008) Blood 111:3442.
  7. Qu, D. et al. (2007) J. Thromb. Haemost. 5:395.
  8. Xu, J. et al. (2000) J. Biol. Chem. 275:6038.
  9. Gur-Cohen, S. et al. (2015) Nat. Med. 21:1307.
  10. Kurosawa, S. et al. (1997) J. Clin. Invest. 100:411.
  11. Liaw, P.C. et al. (2000) J. Biol. Chem. 275:5447.
  12. Villegas-Mendez, A. et al. (2007) J. Thromb. Haemost. 5:980.
  13. Bae, J.-S. et al. (2007) Blood 110:3909.
  14. Fink, K. et al. (2013) PLoS One 8:e53103.
  15. Willcox, C.R. et al. (2012) Nat. Immunol. 13:872.
  16. Turner, L. et al. (2013) Nature 498:502.

Long Name

Endothelial Protein C Receptor

Alternate Names

CCD41, CD201, PROCR

Entrez Gene IDs

10544 (Human); 19124 (Mouse)

Gene Symbol

PROCR

UniProt

AAC42049

Additional EPCR Products

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