Recombinant Mouse Ephrin-A4 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 569-A4

R&D Systems, part of Bio-Techne
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569-A4-200
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Key Product Details

Source

NS0

Accession #

Q3UQB5

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all Ephrin-A4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Ephrin-A4 protein
Mouse Ephrin-A4
(Leu26 - Gly176)
Accession # Q3UQB5		 DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu26

Predicted Molecular Mass

43.6 kDa (monomer)

SDS-PAGE

50-60 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rmEphA7/Fc Chimera at 2 µg/mL (100 µL/well) can bind rmEphrin-A4/Fc Chimera with a linear range of 0.16-10 ng/mL.

Formulation, Preparation and Storage

569-A4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-A4

Ephrin-A4, also known as LERK-4 and EFL-4 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Alternate Names

EFL-4, EFNA4, EphrinA4, LERK-4

Entrez Gene IDs

1945 (Human); 13639 (Mouse)

Gene Symbol

EFNA4

UniProt

Q3UQB5

Additional Ephrin-A4 Products

Product Documents for Recombinant Mouse Ephrin-A4 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse Ephrin-A4 Fc Chimera Protein, CF

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